Identification of genetic and epigenetic similarities of SPHK1/Sphk1 in mammals

Takuya Imamura, Nanami Miyauchi-Senda, Satoshi Tanaka, Kunio Shiota

研究成果: Article

16 引用 (Scopus)

抄録

In normal tissues, methylation of CpG islands is generally accepted to be limited to the inactive X-chromosome and imprinting clusters. Gene Sphk1 has shown complex organization, indicated by multiple alternative splicing and tissue-dependent DNA methylation within the limited area (T-DMR) of the CpG island in the rat. Comparisons among human, mouse and rat SPHK1/Sphk1 genomic DNA revealed five coding exons and association of a CpG island at the 5′ end in common. We also found two novel subtypes, for a total of eight mRNA subtypes generated through selective usage of untranslated first exons. A 38-bp region at the 5′-end of T-DMR is highly conserved. This restricted area is specifically hypomethylated in the brain. Here, we examine the complex genetic/epigenetic features of the SPHK1/Sphk1 CpG island, and suggest that the T-DMR is the core target for tissue-dependent CpG island methylation.

元の言語English
ページ(範囲)1387-1393
ページ数7
ジャーナルJournal of Veterinary Medical Science
66
発行部数11
DOI
出版物ステータスPublished - 2004 11
外部発表Yes

Fingerprint

CpG Islands
Epigenomics
epigenetics
Mammals
mammals
methylation
Methylation
exons
Exons
genomic imprinting
alternative splicing
rats
Alternative Splicing
X Chromosome
X chromosome
DNA methylation
DNA Methylation
brain
genomics
Messenger RNA

ASJC Scopus subject areas

  • veterinary(all)
  • Marketing

これを引用

Identification of genetic and epigenetic similarities of SPHK1/Sphk1 in mammals. / Imamura, Takuya; Miyauchi-Senda, Nanami; Tanaka, Satoshi; Shiota, Kunio.

:: Journal of Veterinary Medical Science, 巻 66, 番号 11, 11.2004, p. 1387-1393.

研究成果: Article

Imamura, Takuya ; Miyauchi-Senda, Nanami ; Tanaka, Satoshi ; Shiota, Kunio. / Identification of genetic and epigenetic similarities of SPHK1/Sphk1 in mammals. :: Journal of Veterinary Medical Science. 2004 ; 巻 66, 番号 11. pp. 1387-1393.
@article{79d191c1433f4ba7ac3e8e0fdd28287d,
title = "Identification of genetic and epigenetic similarities of SPHK1/Sphk1 in mammals",
abstract = "In normal tissues, methylation of CpG islands is generally accepted to be limited to the inactive X-chromosome and imprinting clusters. Gene Sphk1 has shown complex organization, indicated by multiple alternative splicing and tissue-dependent DNA methylation within the limited area (T-DMR) of the CpG island in the rat. Comparisons among human, mouse and rat SPHK1/Sphk1 genomic DNA revealed five coding exons and association of a CpG island at the 5′ end in common. We also found two novel subtypes, for a total of eight mRNA subtypes generated through selective usage of untranslated first exons. A 38-bp region at the 5′-end of T-DMR is highly conserved. This restricted area is specifically hypomethylated in the brain. Here, we examine the complex genetic/epigenetic features of the SPHK1/Sphk1 CpG island, and suggest that the T-DMR is the core target for tissue-dependent CpG island methylation.",
keywords = "Alternative splicing, CpG island, DNA methylation, Sphingosine kinase, Tissue specific",
author = "Takuya Imamura and Nanami Miyauchi-Senda and Satoshi Tanaka and Kunio Shiota",
year = "2004",
month = "11",
doi = "10.1292/jvms.66.1387",
language = "English",
volume = "66",
pages = "1387--1393",
journal = "Journal of Veterinary Medical Science",
issn = "0916-7250",
publisher = "Japanese Society of Veterinary Science",
number = "11",

}

TY - JOUR

T1 - Identification of genetic and epigenetic similarities of SPHK1/Sphk1 in mammals

AU - Imamura, Takuya

AU - Miyauchi-Senda, Nanami

AU - Tanaka, Satoshi

AU - Shiota, Kunio

PY - 2004/11

Y1 - 2004/11

N2 - In normal tissues, methylation of CpG islands is generally accepted to be limited to the inactive X-chromosome and imprinting clusters. Gene Sphk1 has shown complex organization, indicated by multiple alternative splicing and tissue-dependent DNA methylation within the limited area (T-DMR) of the CpG island in the rat. Comparisons among human, mouse and rat SPHK1/Sphk1 genomic DNA revealed five coding exons and association of a CpG island at the 5′ end in common. We also found two novel subtypes, for a total of eight mRNA subtypes generated through selective usage of untranslated first exons. A 38-bp region at the 5′-end of T-DMR is highly conserved. This restricted area is specifically hypomethylated in the brain. Here, we examine the complex genetic/epigenetic features of the SPHK1/Sphk1 CpG island, and suggest that the T-DMR is the core target for tissue-dependent CpG island methylation.

AB - In normal tissues, methylation of CpG islands is generally accepted to be limited to the inactive X-chromosome and imprinting clusters. Gene Sphk1 has shown complex organization, indicated by multiple alternative splicing and tissue-dependent DNA methylation within the limited area (T-DMR) of the CpG island in the rat. Comparisons among human, mouse and rat SPHK1/Sphk1 genomic DNA revealed five coding exons and association of a CpG island at the 5′ end in common. We also found two novel subtypes, for a total of eight mRNA subtypes generated through selective usage of untranslated first exons. A 38-bp region at the 5′-end of T-DMR is highly conserved. This restricted area is specifically hypomethylated in the brain. Here, we examine the complex genetic/epigenetic features of the SPHK1/Sphk1 CpG island, and suggest that the T-DMR is the core target for tissue-dependent CpG island methylation.

KW - Alternative splicing

KW - CpG island

KW - DNA methylation

KW - Sphingosine kinase

KW - Tissue specific

UR - http://www.scopus.com/inward/record.url?scp=16644371208&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16644371208&partnerID=8YFLogxK

U2 - 10.1292/jvms.66.1387

DO - 10.1292/jvms.66.1387

M3 - Article

C2 - 15585953

AN - SCOPUS:16644371208

VL - 66

SP - 1387

EP - 1393

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

SN - 0916-7250

IS - 11

ER -