Identification of genetic and epigenetic similarities of SPHK1/Sphk1 in mammals

Takuya Imamura, Nanami Miyauchi-Senda, Satoshi Tanaka, Kunio Shiota*

*この研究の対応する著者

研究成果: Article査読

17 被引用数 (Scopus)

抄録

In normal tissues, methylation of CpG islands is generally accepted to be limited to the inactive X-chromosome and imprinting clusters. Gene Sphk1 has shown complex organization, indicated by multiple alternative splicing and tissue-dependent DNA methylation within the limited area (T-DMR) of the CpG island in the rat. Comparisons among human, mouse and rat SPHK1/Sphk1 genomic DNA revealed five coding exons and association of a CpG island at the 5′ end in common. We also found two novel subtypes, for a total of eight mRNA subtypes generated through selective usage of untranslated first exons. A 38-bp region at the 5′-end of T-DMR is highly conserved. This restricted area is specifically hypomethylated in the brain. Here, we examine the complex genetic/epigenetic features of the SPHK1/Sphk1 CpG island, and suggest that the T-DMR is the core target for tissue-dependent CpG island methylation.

本文言語English
ページ(範囲)1387-1393
ページ数7
ジャーナルJournal of Veterinary Medical Science
66
11
DOI
出版ステータスPublished - 2004 11月
外部発表はい

ASJC Scopus subject areas

  • 獣医学(全般)
  • マーケティング

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