Identification of novel long chain N-acylhomoserine lactones of chain length c20 from the marine phototrophic bacterium rhodovulum sulfidophilum

Naoko Arashida, Kazutaka Shimbo, Takeshi Terada, Takuya Okimi, Yo Kikuchi, Shuhei Hashiro, So Umekage, Hisashi Yasueda

    研究成果: Article査読

    6 被引用数 (Scopus)

    抄録

    Gram-negative bacterial quorum sensing is mainly regulated by an extracellularly produced N-acylhomoserine lactone (AHL). AHL consists of a lactone ring and an acyl chain, which generally varies from C4 to C18 in length and affords species-specific variety. In this study, we developed an ultra-high performance liquid chromatography tandem mass spectrometry system and detected two kinds of long chain AHLs with chain length C20 from the reverse-phase thin layer chromatography-fractionated cultured supernatant of the marine photosynthetic bacterium Rhodovulum sulfidophilum. By fragmentation search analysis to detect compounds with a homoserine lactone ring moiety for data dependent acquisition, a minor AHL, presumed to be 3-OH-C18-homoserine lactone (HSL), was also found. Among the detected C20-HSLs, 3-OH-C20-HSL was structurally identified and 3-OH-C20:1-HSL was strongly suggested. To our knowledge, this is the first report to show a novel AHL with the longest C20 acyl side chain found to date.

    本文言語English
    ページ(範囲)1683-1693
    ページ数11
    ジャーナルBioscience, Biotechnology and Biochemistry
    82
    10
    DOI
    出版ステータスPublished - 2018 1 1

    ASJC Scopus subject areas

    • バイオテクノロジー
    • 分析化学
    • 生化学
    • 応用微生物学とバイオテクノロジー
    • 分子生物学
    • 有機化学

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    「Identification of novel long chain N-acylhomoserine lactones of chain length c<sub>20</sub> from the marine phototrophic bacterium rhodovulum sulfidophilum」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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