Identification of RNA biomarkers for chemical safety screening in neural cells derived from mouse embryonic stem cells using RNA deep sequencing analysis

Hidenori Tani, Taro Matsutani, Hiroshi Aoki, Kaoru Nakamura, Yu Hamaguchi, Tetsuya Nakazato, Michiaki Hamada

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For chemical safety screening, there is a need for more efficient assays that do not involve animals. Among the alternatives to animal testing, in vitro cell-based assays are among the most appropriate to screen the toxicity of chemicals. Previous studies focused on mRNAs as biomarkers; however, recent whole-transcriptome analyses have revealed the presence of a large number of unannotated noncoding RNAs (ncRNAs) that appear not to have any protein-coding potential. Here, we used deep sequencing analysis (RNA-seq) to identify novel RNA biomarkers, including ncRNAs, that exhibited a substantial response to chemical toxicity from nine chemicals [benzene, bis(2-ethylhexyl)phthalate, chloroform, p-cresol, p-dichlorobenzene, phenol, pyrocatechol, tri-n-butyl phosphate, and trichloroethylene]. We used neural cells derived from mouse embryonic stem cells (mESCs) as a cell-based assay for chemical safety screening. RNA-seq revealed that some ncRNAs responded substantially to the chemical compounds in neural cells derived from mESCs. This indicates that the ncRNAs can be used as novel RNA biomarkers for chemical safety screening.

元の言語English
ジャーナルBiochemical and Biophysical Research Communications
DOI
出版物ステータスAccepted/In press - 2018 1 1

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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