@article{de96d37c8b204cad8822b11dcec9e507,
title = "Identification of zinc finger transcription factor EGR2 as a novel acetylated protein",
abstract = "EGR2 is a zinc finger transcription factor that regulates myelination in the peripheral nervous system and T cell anergy. The transcriptional activity of EGR2 is known to be regulated by its co-activators and/or co-repressors. Although the activity of transcription factors is generally regulated not only by interactions with co-regulators but also posttranslational modifications including acetylation, little is known about posttranslational modifications of EGR2. Here we show that EGR2 is a novel acetylated protein. Through immunoblotting analyses using an antibody that specifically recognizes the acetylated form of EGR2, CBP and p300 were identified as acetyltransferases, while HDAC6, 10 and SIRT1 were identified as deacetylases of EGR2. Although the NuRD complex containing HDAC1 and HDAC2 is known to associate with EGR2, the present study suggests that acetylation of EGR2 is regulated independently of NuRD.",
keywords = "Acetylation, EGR2, HAT, HDAC",
author = "Kota Noritsugu and Akihiro Ito and Yoichi Nakao and Minoru Yoshida",
note = "Funding Information: We thank Kaori Otsuki, Masaya Usui, and Aya Abe (Support Unit for Bio-Material Analysis, RIKEN BSI Research Resources Center) for technical help with MS analysis, the staff of RRC for peptide synthesis, antibody production, DNA sequencing and providing FANTOM clone, and Elliot Bradshaw for proofreading. This work was supported by the Japan Society for the Promotion of Science under Grants-in-Aid for Scientific Research (S) (26221204) and for Challenging Exploratory Research (16K14674) and supported by RIKEN Junior Research Associate Program. This work was inspired by the JSPS Asian Chemical Biology Initiative. Publisher Copyright: {\textcopyright} 2017 Elsevier Inc. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",
year = "2017",
month = aug,
day = "5",
doi = "10.1016/j.bbrc.2017.05.170",
language = "English",
volume = "489",
pages = "455--459",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",
}