Imaging phospholipid conformational disorder and packing in giant multilamellar liposome by confocal Raman microspectroscopy

Hemanth Noothalapati, Keita Iwasaki, Chikako Yoshimoto, Keisuke Yoshikiyo, Tomoe Nishikawa, Masahiro Ando, Hiro o. Hamaguchi, Tatsuyuki Yamamoto

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Liposomes are closed phospholipid bilayer systems that have profound applications in fundamental cell biology, pharmaceutics and medicine. Depending on the composition (pure or mixture of phospholipids, presence of cholesterol) and preparation protocol, intra- and inter-chain molecular interactions vary leading to changes in the quality (order and packing) of liposomes. So far it is not possible to image conformational disorders and packing densities within a liposome in a straightforward manner. In this study, we utilized confocal Raman microspectroscopy to visualize structural disorders and packing efficiency within a giant multilamellar liposome model by focusing mainly on three regions in the vibrational spectrum (C[sbnd]C stretching, C[sbnd]H deformation and C[sbnd]H stretching). We estimated properties such as trans/gauche isomers and lateral packing probability. Interestingly, our Raman imaging studies revealed gel phase rich domains and heterogeneous lateral packing within the giant multilamellar liposome.

本文言語English
ページ(範囲)186-190
ページ数5
ジャーナルSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
187
DOI
出版ステータスPublished - 2017 12 5

ASJC Scopus subject areas

  • 分析化学
  • 原子分子物理学および光学
  • 器械工学
  • 分光学

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