Imaging the lateral diffusion of membrane molecules with quantum dots

Hiroko Bannai, Sabine Lévi, Claude Schweizer, Maxime Dahan, Antoine Triller*

*この研究の対応する著者

研究成果: Article査読

126 被引用数 (Scopus)

抄録

This protocol describes a sensitive approach to tracking the motion of membrane molecules such as lipids and proteins with molecular resolution in live cells. This technique makes use of fluorescent semiconductor nanocrystals, quantum dots (QDs), as a probe to detect membrane molecules of interest. The photostability and brightness of QDs allow them to be tracked at a single particle level for longer periods than previous fluorophores, such as fluorescent proteins and organic dyes. QDs are bound to the extracellular part of the object to be followed, and their movements can be recorded with a fluorescence microscope equipped with a spectral lamp and a sensitive cooled charge-coupled device camera. The experimental procedure described for neurons below takes about 45 min. This technique is applicable to various cultured cells.

本文言語English
ページ(範囲)2628-2634
ページ数7
ジャーナルNature protocols
1
6
DOI
出版ステータスPublished - 2007 1
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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