Immobilized antibody-based flow type enzyme immunosensor for determination of human serum albumin

Isao Karube, Tadashi Matsunaga, Shuichi Suzuki, Taichi Asano, Satoshi Itoh

研究成果: Article

3 引用 (Scopus)

抄録

A flow-type enzyme immunosensor was prepared for the electrochemical determination of human serum albumin (HSA). The immunosensor was constructed from the immobilized antibody (anti-HSA IgG) reactor and an oxygen electrode. The immunochemical reaction of catalase-labelled antibody with HSA was completed with 30 min. After the immunochemical reaction, hydrogen peroxide solution was injected into the system and a peak current was obtained within 2 min. A linear relationship was observed between the current increase and the logarithm of HSA concentration in the range 10-8-10-6 g ml-1. The minimum measurable concentration was 10-8 g ml-1. The current increase was reproducible with 10% of the relative errors when a sample solution containing 10-7 g ml-1 of HSA was used. The minimum measurable concentration increased to 10-9 g ml-1 when hydrogen peroxide was recycled for 5 min in the reactor system. The immobilized antibody reactor could be reused. HSA in human serum was determined by the system proposed.

元の言語English
ページ(範囲)279-286
ページ数8
ジャーナルJournal of Biotechnology
1
発行部数5-6
DOI
出版物ステータスPublished - 1984
外部発表Yes

Fingerprint

Immobilized Antibodies
Immunosensors
Antibodies
Serum Albumin
Enzymes
Hydrogen peroxide
Hydrogen Peroxide
Electrodes
Oxygen
Catalase
Immunoglobulin G
Serum

ASJC Scopus subject areas

  • Biotechnology

これを引用

Immobilized antibody-based flow type enzyme immunosensor for determination of human serum albumin. / Karube, Isao; Matsunaga, Tadashi; Suzuki, Shuichi; Asano, Taichi; Itoh, Satoshi.

:: Journal of Biotechnology, 巻 1, 番号 5-6, 1984, p. 279-286.

研究成果: Article

Karube, Isao ; Matsunaga, Tadashi ; Suzuki, Shuichi ; Asano, Taichi ; Itoh, Satoshi. / Immobilized antibody-based flow type enzyme immunosensor for determination of human serum albumin. :: Journal of Biotechnology. 1984 ; 巻 1, 番号 5-6. pp. 279-286.
@article{839cc774ff6643c6b1eed8dcef2c6299,
title = "Immobilized antibody-based flow type enzyme immunosensor for determination of human serum albumin",
abstract = "A flow-type enzyme immunosensor was prepared for the electrochemical determination of human serum albumin (HSA). The immunosensor was constructed from the immobilized antibody (anti-HSA IgG) reactor and an oxygen electrode. The immunochemical reaction of catalase-labelled antibody with HSA was completed with 30 min. After the immunochemical reaction, hydrogen peroxide solution was injected into the system and a peak current was obtained within 2 min. A linear relationship was observed between the current increase and the logarithm of HSA concentration in the range 10-8-10-6 g ml-1. The minimum measurable concentration was 10-8 g ml-1. The current increase was reproducible with 10{\%} of the relative errors when a sample solution containing 10-7 g ml-1 of HSA was used. The minimum measurable concentration increased to 10-9 g ml-1 when hydrogen peroxide was recycled for 5 min in the reactor system. The immobilized antibody reactor could be reused. HSA in human serum was determined by the system proposed.",
keywords = "flow-type immunoreactor, human serum albumin, immobilized antibody, immunosensor",
author = "Isao Karube and Tadashi Matsunaga and Shuichi Suzuki and Taichi Asano and Satoshi Itoh",
year = "1984",
doi = "10.1016/0168-1656(84)90019-1",
language = "English",
volume = "1",
pages = "279--286",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",
number = "5-6",

}

TY - JOUR

T1 - Immobilized antibody-based flow type enzyme immunosensor for determination of human serum albumin

AU - Karube, Isao

AU - Matsunaga, Tadashi

AU - Suzuki, Shuichi

AU - Asano, Taichi

AU - Itoh, Satoshi

PY - 1984

Y1 - 1984

N2 - A flow-type enzyme immunosensor was prepared for the electrochemical determination of human serum albumin (HSA). The immunosensor was constructed from the immobilized antibody (anti-HSA IgG) reactor and an oxygen electrode. The immunochemical reaction of catalase-labelled antibody with HSA was completed with 30 min. After the immunochemical reaction, hydrogen peroxide solution was injected into the system and a peak current was obtained within 2 min. A linear relationship was observed between the current increase and the logarithm of HSA concentration in the range 10-8-10-6 g ml-1. The minimum measurable concentration was 10-8 g ml-1. The current increase was reproducible with 10% of the relative errors when a sample solution containing 10-7 g ml-1 of HSA was used. The minimum measurable concentration increased to 10-9 g ml-1 when hydrogen peroxide was recycled for 5 min in the reactor system. The immobilized antibody reactor could be reused. HSA in human serum was determined by the system proposed.

AB - A flow-type enzyme immunosensor was prepared for the electrochemical determination of human serum albumin (HSA). The immunosensor was constructed from the immobilized antibody (anti-HSA IgG) reactor and an oxygen electrode. The immunochemical reaction of catalase-labelled antibody with HSA was completed with 30 min. After the immunochemical reaction, hydrogen peroxide solution was injected into the system and a peak current was obtained within 2 min. A linear relationship was observed between the current increase and the logarithm of HSA concentration in the range 10-8-10-6 g ml-1. The minimum measurable concentration was 10-8 g ml-1. The current increase was reproducible with 10% of the relative errors when a sample solution containing 10-7 g ml-1 of HSA was used. The minimum measurable concentration increased to 10-9 g ml-1 when hydrogen peroxide was recycled for 5 min in the reactor system. The immobilized antibody reactor could be reused. HSA in human serum was determined by the system proposed.

KW - flow-type immunoreactor

KW - human serum albumin

KW - immobilized antibody

KW - immunosensor

UR - http://www.scopus.com/inward/record.url?scp=0021723340&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021723340&partnerID=8YFLogxK

U2 - 10.1016/0168-1656(84)90019-1

DO - 10.1016/0168-1656(84)90019-1

M3 - Article

AN - SCOPUS:0021723340

VL - 1

SP - 279

EP - 286

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 5-6

ER -