Immuno-capture of Cryptosporidium parvum using micro-well array

Tomoyuki Taguchi, Haruko Takeyama, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

14 被引用数 (Scopus)

抄録

A glass slide and micro-well array chip on which anti-Cryptosporidium parvum antibody was immobilized were used for the rapid capture and detection of C. parvum. Biotinylated anti-C. parvum antibodies were spotted onto the streptavidin-coated glass slides. C. parvum oocysts were captured specifically on the spot when more than 73 ng of anti-C. parvum antibody was applied onto the glass slide. However, C. parvum oocysts captured on the glass slide were detached by repeating washing steps. To improve the capture efficiency of oocysts, capture was performed in a micro-well format consisting of 1024 wells/2.5 mm2 (32 × 32 wells) fabricated as a chip by photolithography. Instead of a flat surface on a glass slide, each well was 30 μm in diameter and 10 μm in depth. Streptavidin was also immobilized onto the micro-well array. The biotinylated anti-C. parvum antibodies were immobilized efficiently onto the chip using a buffer containing 20% methanol. Using this technique C. parvum oocysts were stably captured onto the chip after repeated washing procedures. These data show that the newly designed micro-well array technique described here is useful for antibody-mediated C. parvum capture.

本文言語English
ページ(範囲)2276-2282
ページ数7
ジャーナルBiosensors and Bioelectronics
20
11
DOI
出版ステータスPublished - 2005 5 15
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生物理学
  • 生体医工学
  • 電気化学

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