抄録
Extraretinal photoreceptor cells have been found in the pineal complex and deep brain of a variety of non-mammalian vertebrates. Light signals received by these photoreceptor cells seem to be a potent regulator of diverse physiological responses. Here, the pineal complex and deep brain of the Japanese grass lizard, Takydromus tachydromoides, were immunohistochemically analyzed to localize the photoreceptive molecule (opsin) and the light signal-transducing G-protein (transducin). In addition to the pineal organ and parietal eye constituting the pineal complex, we unexpectedly found a parapineal organ, which is located just below the parietal eye and is morphologically similar to the pineal organ. Both organs had photoreceptor-like cells with outer segments immunostained by anti-rhodopsin and anti-pinopsin antibodies. Neither opsin- nor transducin-like immunoreactivities were detected in the parietal eye with all the antibodies tested in this study, although its morphology resembles that of the lateral eyes. In the deep brain region, rhodopsin-like immunoreactivities were observed in the posterior palliai commissure and median eminence. The cerebrospinal fluid-contacting neurons in the paraventricular organ were immunoreactive to an antibody against α-subunit of cone transducin. In lizards, this is the first report showing (1) rhodopsin- and pinopsin-like immunoreactivities in the parapineal organ, (2) rhodopsin-like immunoreactivity in the deep brain, and (3) putative photoreceptive areas in the hypothalamus.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 325-330 |
| ページ数 | 6 |
| ジャーナル | Zoological Science |
| 巻 | 18 |
| 発行部数 | 3 |
| DOI | |
| 出版物ステータス | Published - 2001 4 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Animal Science and Zoology
これを引用
Immunohistochemical localization of opsins and alpha-subunit of transducin in the pineal complex and deep brain of the Japanese grass lizard, Takydromus tachydromoides. / Yoshikawa, Tomoko; Okano, Toshiyuki; Kokame, Koichi; Hisatomi, Osamu; Tokunaga, Fumio; Oishi, Tadashi; Fukada, Yoshitaka.
：: Zoological Science, 巻 18, 番号 3, 04.2001, p. 325-330.研究成果: Article
}
TY - JOUR
T1 - Immunohistochemical localization of opsins and alpha-subunit of transducin in the pineal complex and deep brain of the Japanese grass lizard, Takydromus tachydromoides
AU - Yoshikawa, Tomoko
AU - Okano, Toshiyuki
AU - Kokame, Koichi
AU - Hisatomi, Osamu
AU - Tokunaga, Fumio
AU - Oishi, Tadashi
AU - Fukada, Yoshitaka
PY - 2001/4
Y1 - 2001/4
N2 - Extraretinal photoreceptor cells have been found in the pineal complex and deep brain of a variety of non-mammalian vertebrates. Light signals received by these photoreceptor cells seem to be a potent regulator of diverse physiological responses. Here, the pineal complex and deep brain of the Japanese grass lizard, Takydromus tachydromoides, were immunohistochemically analyzed to localize the photoreceptive molecule (opsin) and the light signal-transducing G-protein (transducin). In addition to the pineal organ and parietal eye constituting the pineal complex, we unexpectedly found a parapineal organ, which is located just below the parietal eye and is morphologically similar to the pineal organ. Both organs had photoreceptor-like cells with outer segments immunostained by anti-rhodopsin and anti-pinopsin antibodies. Neither opsin- nor transducin-like immunoreactivities were detected in the parietal eye with all the antibodies tested in this study, although its morphology resembles that of the lateral eyes. In the deep brain region, rhodopsin-like immunoreactivities were observed in the posterior palliai commissure and median eminence. The cerebrospinal fluid-contacting neurons in the paraventricular organ were immunoreactive to an antibody against α-subunit of cone transducin. In lizards, this is the first report showing (1) rhodopsin- and pinopsin-like immunoreactivities in the parapineal organ, (2) rhodopsin-like immunoreactivity in the deep brain, and (3) putative photoreceptive areas in the hypothalamus.
AB - Extraretinal photoreceptor cells have been found in the pineal complex and deep brain of a variety of non-mammalian vertebrates. Light signals received by these photoreceptor cells seem to be a potent regulator of diverse physiological responses. Here, the pineal complex and deep brain of the Japanese grass lizard, Takydromus tachydromoides, were immunohistochemically analyzed to localize the photoreceptive molecule (opsin) and the light signal-transducing G-protein (transducin). In addition to the pineal organ and parietal eye constituting the pineal complex, we unexpectedly found a parapineal organ, which is located just below the parietal eye and is morphologically similar to the pineal organ. Both organs had photoreceptor-like cells with outer segments immunostained by anti-rhodopsin and anti-pinopsin antibodies. Neither opsin- nor transducin-like immunoreactivities were detected in the parietal eye with all the antibodies tested in this study, although its morphology resembles that of the lateral eyes. In the deep brain region, rhodopsin-like immunoreactivities were observed in the posterior palliai commissure and median eminence. The cerebrospinal fluid-contacting neurons in the paraventricular organ were immunoreactive to an antibody against α-subunit of cone transducin. In lizards, this is the first report showing (1) rhodopsin- and pinopsin-like immunoreactivities in the parapineal organ, (2) rhodopsin-like immunoreactivity in the deep brain, and (3) putative photoreceptive areas in the hypothalamus.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035532904&partnerID=8YFLogxK
U2 - 10.2108/zsj.18.325
DO - 10.2108/zsj.18.325
M3 - Article
AN - SCOPUS:0035532904
VL - 18
SP - 325
EP - 330
JO - Zoological Science
JF - Zoological Science
SN - 0289-0003
IS - 3
ER -