Immunoreactive insulin in diabetes mellitus patient sera detected by ultrasensitive ELISA with thio-NAD cycling

Etsuro Ito, Mugiho Kaneda, Hiromi Kodama, Mika Morikawa, Momoko Tai, Kana Aoki, Satoshi Watabe, Kazunari Nakaishi, Seiichi Hashida, Satoshi Tada, Noriyuki Kuroda, Hitomi Imachi, Koji Murao, Masakane Yamashita, Teruki Yoshimura, Toshiaki Miura

研究成果: Article

8 被引用数 (Scopus)

抄録

To minimize patient suffering, the smallest possible volume of blood should be collected for diagnosis and disease monitoring. When estimating insulin secretion capacity and resistance to insulin in diabetes mellitus (DM), increasing insulin assay immunosensitivity would reduce the blood sample volume required for testing. Here we present an ultrasensitive ELISA coupled with thio-NAD cycling to measure immunoreactive insulin in blood serum. Only 5 mL of serum was required for testing, with a limit of detection (LOD) for the assay of 10-16 moles/assay. Additional recovery tests confirmed this method can detect insulin in sera. Comparisons between a commercially available immunoreactive insulin kit and our ultrasensitive ELISA using the same commercially available reference demonstrated good data correlation, providing further evidence of assay accuracy. Together, these results demonstrate our ultrasensitive ELISA could be a powerful tool in the diagnosis and treatment of not only DM but also many other diseases in the future.

本文言語English
論文番号114355
ページ(範囲)359-367
ページ数9
ジャーナルBioTechniques
59
6
DOI
出版ステータスPublished - 2015 12

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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