Impaired Photosystem II in a Mutant of Chlamydomonas Reinhardtii Defective in Sulfoquinovosyl Diacylglycerol

Norihiro Sato, Kintake Sonoike, Mikio Tsuzuk, Akihiko Kawaguchi

研究成果: Article査読

77 被引用数 (Scopus)

抄録

The photosynthetic apparatus was characterized in a mutant of Chlamydomonas reinhardtii, hf‐2, defective in the synthesis of a chloroplast‐specific lipid, sulfoquinovosyl diacylglycerol (SQui‐acyl2Gro). hf ‐2 showed reduced photosystem II (PSII) activity with little effect on photosystem I (PSI) activity, as compared with the parent. PAGE in the presence of dodecyl β‐D‐maltoside (DodGlc2) of C. reinhardtii thylakoid membranes was used to isolate chlorophyll‐protein complexes without chlorophyll (Chi) release in order to examine lipid species bound to these complexes. The four complexes obtained were shown to be the PSI complex, the PSII core complex and the two groups of the light‐harvesting complex of PSII by analyses of 77‐K emission spectra of Chi fluorescence and of subunit compositions. Lipid analysis of Chl‐protein complexes in the parent revealed the localization of SQui‐acyl2Gro in the PSII core complex and the two groups of the light‐harvesting complex of PSII, but not in the PSI complex. These results suggest that SQui‐acyl2Gro is responsible for PSII activity by associating with the core and light‐harvesting complexes of PSII.

本文言語English
ページ(範囲)16-23
ページ数8
ジャーナルEuropean Journal of Biochemistry
234
1
DOI
出版ステータスPublished - 1995 11
外部発表はい

ASJC Scopus subject areas

  • Biochemistry

フィンガープリント 「Impaired Photosystem II in a Mutant of Chlamydomonas Reinhardtii Defective in Sulfoquinovosyl Diacylglycerol」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル