Improvement of oxygen carrying ability of hemoglobin vesicles by H 2O 2 elimination system

Tomoyasu Atoji, Motonari Aihara, Shinji Takeoka, Eishun Tsuchida

研究成果: Paper査読

抄録

Oxyhemoglobin (oxyHb) of Hb-based oxygen carriers reacts with hydrogen peroxide (H 2O 2) to convert metHb and loses its oxygen binding ability. We have developed Hb-vesicles which encapsulate concentrated Hb with phospholipids bilayer membrane. Here, we constructed a suppression system of metHb formation within Hb vesicles using the peroxidase activity of metHb with L-Tyr. We could suppress the metHb formation of the oxyHb in the Hb vesicles by coencapsulating metHb and L-Tyr on the experiment of stepwise addition of H 2O 2 and autoxidation condition. Furthermore, this system was also effective in vivo. We concluded that H 2O 2 was effectively eliminated by the metHb/L-Tyr system in the Hb vesicles, and oxygen carrying ability was successfully prolonged.

本文言語English
ページ4927-4928
ページ数2
出版ステータスPublished - 2005 12 1
イベント54th SPSJ Symposium on Macromolecules - Yamagata, Japan
継続期間: 2005 9 202005 9 22

Other

Other54th SPSJ Symposium on Macromolecules
国/地域Japan
CityYamagata
Period05/9/2005/9/22

ASJC Scopus subject areas

  • 工学(全般)

フィンガープリント

「Improvement of oxygen carrying ability of hemoglobin vesicles by H <sub>2</sub>O <sub>2</sub> elimination system」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル