Improvement of protein binding capacity of acrylic-acid-grafted fibers by polymer root-to-brush shift

Yuka Matsuzaki, Takeshi Itabashi, Shigeko Kawai-Noma, Daisuke Umeno, Kyoichi Saito*

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

A weak acid cation exchange fiber used for large-scale antibody purification was prepared by radiation-induced graft polymerization of acrylic acid (AA) onto a commercially available nylon-6 fiber. The AA-grafted fiber was post-treated by conditioning in NaOH aqueous solution at 298 K followed by immersion in water at 353 K to increase the protein binding capacity of the cation exchange fiber. A 2 g/L lysozyme solution buffered at pH 6.0 flowed through a column charged with a treated or untreated AA-grafted fiber at a space velocity of 20 h −1 . The 10% dynamic binding capacity of the column charged with the former fiber of 200 mg/mL-column was 5.9-fold that of the column charged with the latter fiber, and 2.3-fold that (88 mg/mL-column) of a column charged with CM Sepharose™ Fast Flow (GE Healthcare).

本文言語English
ページ(範囲)131-136
ページ数6
ジャーナルRadiation Physics and Chemistry
158
DOI
出版ステータスPublished - 2019 5
外部発表はい

ASJC Scopus subject areas

  • 放射線

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