Improvement of the aluminum borate whisker-mediated method of DNA delivery into rice callus

Kiyotaka Mizuno, Wataru Takahashi, Takashi Ohyama, Takiko Shimada, Osamu Tanaka*

*この研究の対応する著者

研究成果: Article査読

14 被引用数 (Scopus)

抄録

We improved the aluminum borate whisker-mediated method of DNA delivery into rice callus (Oryza saliva L., cv. Notohikari). The following factors were examined: amount of whiskers, the kind of apparatus for agitation, the type of whiskers, duration of agitation, and agitation speed. Twenty callused scutellar tissues were agitated in a 1.5 ml microtube containing aluminum borate whiskers (ABW), liquid medium, and the plasmid pAct1-F carrying the β-glucuronidase (GUS) gene. After the agitation, the scutellar tissues were subjected to transient GUS expression assay, which visualizes GUS-expressing cells as blue spots. Efficiencies of DNA delivery were evaluated as the number of blue spots resulting from the assay. In the present study, we succeeded in improving the efficiency of DNA delivery by changing the apparatus for agitation from rotary (Vortex Genie 2) to multidirectional (MT-360), and the type of ABW from Alborex Y to an aminosilane-coated Alborex (Alborex YS3A).

本文言語English
ページ(範囲)45-49
ページ数5
ジャーナルPlant Production Science
7
1
DOI
出版ステータスPublished - 2004 3
外部発表はい

ASJC Scopus subject areas

  • 農業および作物学

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