In-situ guidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers

Hideaki Yamamoto, Kazunori Okano, Takanori Demura, Yoichiroh Hosokawa, Hiroshi Masuhara, Takashi Tanii, Shun Nakamura

研究成果: Article査読

25 被引用数 (Scopus)

抄録

In-situ guidance of neuronal processes (neurites) is demonstrated by applying wet femtosecond-laser processing to an organosilane self-assembled monolayer (SAM) template. By scanning focused laser beam between cell adhesion sites, on which primary neurons adhered and extended their neurites, we succeeded in guiding the neurites along the laser-scanning line. This guidance was accomplished by multiphoton laser ablation of cytophobic SAM layer and subsequent adsorption of cell adhesion molecule, laminin, onto the ablated region. This technique allows us to arbitrarily design neuronal networks in vitro.

本文言語English
論文番号163701
ジャーナルApplied Physics Letters
99
16
DOI
出版ステータスPublished - 2011 10 17

ASJC Scopus subject areas

  • 物理学および天文学(その他)

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