In situ PCR for visualizing distribution of a functional gene "amoA" in a biofilm regardless of activity

Tatsuhiko Hoshino, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

    研究成果: Article

    9 引用 (Scopus)

    抄録

    In this study, ammonia-oxidizing bacteria present in biofilms resulting from a nitrifying reactor were detected by both a conventional FISH technique and an original in situ PCR technique. Both techniques showed that ammonia-oxidizing bacteria were found near the surface of the biofilms. However, after the biofilm had been exposed to 2 weeks of ammonia starvation, ammonia-oxidizing bacteria present in the biofilm could not be detected by fluorescence in situ hybridization (FISH) because they did not have sufficient copies of rRNA. In contrast, ammonia-oxidizing bacteria could be detected by in situ PCR with strong signal. It was thus demonstrated that a cell possessing a specific functional gene is detectable by in situ PCR regardless of its activity.

    元の言語English
    ページ(範囲)33-40
    ページ数8
    ジャーナルJournal of Biotechnology
    105
    発行部数1-2
    DOI
    出版物ステータスPublished - 2003 10 9

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    Biofilms
    Ammonia
    Genes
    Bacteria
    Polymerase Chain Reaction
    Fluorescence In Situ Hybridization
    Fluorescence
    Starvation

    ASJC Scopus subject areas

    • Biotechnology

    これを引用

    In situ PCR for visualizing distribution of a functional gene "amoA" in a biofilm regardless of activity. / Hoshino, Tatsuhiko; Tsuneda, Satoshi; Hirata, Akira; Inamori, Yuhei.

    :: Journal of Biotechnology, 巻 105, 番号 1-2, 09.10.2003, p. 33-40.

    研究成果: Article

    Hoshino, Tatsuhiko ; Tsuneda, Satoshi ; Hirata, Akira ; Inamori, Yuhei. / In situ PCR for visualizing distribution of a functional gene "amoA" in a biofilm regardless of activity. :: Journal of Biotechnology. 2003 ; 巻 105, 番号 1-2. pp. 33-40.
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    abstract = "In this study, ammonia-oxidizing bacteria present in biofilms resulting from a nitrifying reactor were detected by both a conventional FISH technique and an original in situ PCR technique. Both techniques showed that ammonia-oxidizing bacteria were found near the surface of the biofilms. However, after the biofilm had been exposed to 2 weeks of ammonia starvation, ammonia-oxidizing bacteria present in the biofilm could not be detected by fluorescence in situ hybridization (FISH) because they did not have sufficient copies of rRNA. In contrast, ammonia-oxidizing bacteria could be detected by in situ PCR with strong signal. It was thus demonstrated that a cell possessing a specific functional gene is detectable by in situ PCR regardless of its activity.",
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    AU - Inamori, Yuhei

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