In situ telomerase activity in pleural effusions: A promising marker for malignancy

Annika Dejmek, Naoyuki Yahata, Kazuma Ohyashiki, Yoshiro Ebihara, Masatoshi Kakihana, Takashi Hirano, Norihiko Kawate, Harubumi Kato

研究成果: Article

25 引用 (Scopus)

抄録

Telomerase activity in 16 pleural effusions was studied using an in situ telomerase repeat amplification protocol (TRAP) assay on cytospin preparations. Six of nine cytologically malignant specimens contained telomerase-positive cells (67%), and in two further specimens, suspicious positive cells were seen. Two of four atypical specimens contained telomerase-positive cells, whereas two benign cases were telomerase-negative. No mesothelial cells showed telomerase reactivity. Thus, telomerase activity was specific for malignancy and it was always found only in malignant cells. The results suggest that telomerase activity measured with this in situ method can be a valuable complement in the assessment of malignancy in pleural effusions. Diagn. Cytopathol. 2001;24: 11-15.

元の言語English
ページ(範囲)11-15
ページ数5
ジャーナルDiagnostic Cytopathology
24
発行部数1
DOI
出版物ステータスPublished - 2001
外部発表Yes

Fingerprint

Telomerase
Pleural Effusion
Neoplasms

ASJC Scopus subject areas

  • Anatomy

これを引用

In situ telomerase activity in pleural effusions : A promising marker for malignancy. / Dejmek, Annika; Yahata, Naoyuki; Ohyashiki, Kazuma; Ebihara, Yoshiro; Kakihana, Masatoshi; Hirano, Takashi; Kawate, Norihiko; Kato, Harubumi.

:: Diagnostic Cytopathology, 巻 24, 番号 1, 2001, p. 11-15.

研究成果: Article

Dejmek, Annika ; Yahata, Naoyuki ; Ohyashiki, Kazuma ; Ebihara, Yoshiro ; Kakihana, Masatoshi ; Hirano, Takashi ; Kawate, Norihiko ; Kato, Harubumi. / In situ telomerase activity in pleural effusions : A promising marker for malignancy. :: Diagnostic Cytopathology. 2001 ; 巻 24, 番号 1. pp. 11-15.
@article{8b321b48b6d14afa8d33312e7f057136,
title = "In situ telomerase activity in pleural effusions: A promising marker for malignancy",
abstract = "Telomerase activity in 16 pleural effusions was studied using an in situ telomerase repeat amplification protocol (TRAP) assay on cytospin preparations. Six of nine cytologically malignant specimens contained telomerase-positive cells (67{\%}), and in two further specimens, suspicious positive cells were seen. Two of four atypical specimens contained telomerase-positive cells, whereas two benign cases were telomerase-negative. No mesothelial cells showed telomerase reactivity. Thus, telomerase activity was specific for malignancy and it was always found only in malignant cells. The results suggest that telomerase activity measured with this in situ method can be a valuable complement in the assessment of malignancy in pleural effusions. Diagn. Cytopathol. 2001;24: 11-15.",
keywords = "Cytology, Effusions, Telomerase, TRAP in situ",
author = "Annika Dejmek and Naoyuki Yahata and Kazuma Ohyashiki and Yoshiro Ebihara and Masatoshi Kakihana and Takashi Hirano and Norihiko Kawate and Harubumi Kato",
year = "2001",
doi = "10.1002/1097-0339(200101)24:1<11::AID-DC1001>3.0.CO;2-A",
language = "English",
volume = "24",
pages = "11--15",
journal = "Diagnostic Cytopathology",
issn = "8755-1039",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - In situ telomerase activity in pleural effusions

T2 - A promising marker for malignancy

AU - Dejmek, Annika

AU - Yahata, Naoyuki

AU - Ohyashiki, Kazuma

AU - Ebihara, Yoshiro

AU - Kakihana, Masatoshi

AU - Hirano, Takashi

AU - Kawate, Norihiko

AU - Kato, Harubumi

PY - 2001

Y1 - 2001

N2 - Telomerase activity in 16 pleural effusions was studied using an in situ telomerase repeat amplification protocol (TRAP) assay on cytospin preparations. Six of nine cytologically malignant specimens contained telomerase-positive cells (67%), and in two further specimens, suspicious positive cells were seen. Two of four atypical specimens contained telomerase-positive cells, whereas two benign cases were telomerase-negative. No mesothelial cells showed telomerase reactivity. Thus, telomerase activity was specific for malignancy and it was always found only in malignant cells. The results suggest that telomerase activity measured with this in situ method can be a valuable complement in the assessment of malignancy in pleural effusions. Diagn. Cytopathol. 2001;24: 11-15.

AB - Telomerase activity in 16 pleural effusions was studied using an in situ telomerase repeat amplification protocol (TRAP) assay on cytospin preparations. Six of nine cytologically malignant specimens contained telomerase-positive cells (67%), and in two further specimens, suspicious positive cells were seen. Two of four atypical specimens contained telomerase-positive cells, whereas two benign cases were telomerase-negative. No mesothelial cells showed telomerase reactivity. Thus, telomerase activity was specific for malignancy and it was always found only in malignant cells. The results suggest that telomerase activity measured with this in situ method can be a valuable complement in the assessment of malignancy in pleural effusions. Diagn. Cytopathol. 2001;24: 11-15.

KW - Cytology

KW - Effusions

KW - Telomerase

KW - TRAP in situ

UR - http://www.scopus.com/inward/record.url?scp=0035160611&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035160611&partnerID=8YFLogxK

U2 - 10.1002/1097-0339(200101)24:1<11::AID-DC1001>3.0.CO;2-A

DO - 10.1002/1097-0339(200101)24:1<11::AID-DC1001>3.0.CO;2-A

M3 - Article

C2 - 11135462

AN - SCOPUS:0035160611

VL - 24

SP - 11

EP - 15

JO - Diagnostic Cytopathology

JF - Diagnostic Cytopathology

SN - 8755-1039

IS - 1

ER -