In vitro activity of the hairpin ribozyme derived from the negative strand of arabis mosaic virus satellite RNA

Shin Hisamatsu, Yuki Morikawa, Rie Tomita, Terumichi Tanaka, Shigenori Sonoki, Yo Kikuchi

研究成果: Article査読

1 被引用数 (Scopus)

抄録

The negative strand of the satellite RNA of tobacco ringspot virus [(-)sTRSV] is a self-cleaving RNA, of which self-cleaving domain is called the hairpin ribozyme. The negative strand of the satellite RNA of arabis mosaic virus [(-)sArMV] has been suggested to have a hairpin ribozyme-like secondary structure, and we have previously shown that this hairpin domain of(-)sArMV has ribozyme activity. Here we report characterization of the cleavage reaction of the (-)sArMV hairpin ribozyme. Mutagenesis analyses in a transacting system revealed, surprisingly, that the wild-type ribozyme was less active than almost all the other mutant ribozymes tested. In a cis-acting system (self-cleaving reaction), however, the reaction of the RNA containing the wild-type sequence proceeds highly efficiently. This result suggests that the inefficient cleavage of the wild-type substrate in trans-acting system may be due to low efficiency at the substrate-binding step but not at the chemical cleavage step in the reaction. We also constructed a chimeric ribozyme between the catalytic hairpin domain from (-)sArMV and the substrate-binding site from (-)sTRSV. This chimeric ribozyme had the highest activity among the traits-acting hairpin ribozymes tested.

本文言語English
ページ(範囲)352-357
ページ数6
ジャーナルJournal of Biochemistry
122
2
出版ステータスPublished - 1997 8
外部発表はい

ASJC Scopus subject areas

  • Biochemistry

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