In vitro engineering of vascularized tissue surrogates

Katsuhisa Sakaguchi, Tatsuya Shimizu, Shigeto Horaguchi, Hidekazu Sekine, Masayuki Yamato, Mitsuo Umezu, Teruo Okano

研究成果: Article

181 引用 (Scopus)

抜粋

In vitro scaling up of bioengineered tissues is known to be limited by diffusion issues, specifically a lack of vasculature. Here, we report a new strategy for preserving cell viability in three-dimensional tissues using cell sheet technology and a perfusion bioreactor having collagen-based microchannels. When triple-layer cardiac cell sheets are incubated within this bioreactor, endothelial cells in the cell sheets migrate to vascularize in the collagen gel, and finally connect with the microchannels. Medium readily flows into the cell sheets through the microchannels and the newly developed capillaries, while the cardiac construct shows simultaneous beating. When additional triple-layer cell sheets are repeatedly layered, new multi-layer construct spontaneously integrates and the resulting construct becomes a vascularized thick tissue. These results confirmed our method to fabricate in vitro vascularized tissue surrogates that overcomes engineered-tissue thickness limitations. The surrogates promise new therapies for damaged organs as well as new in vitro tissue models.

元の言語English
記事番号1316
ジャーナルScientific reports
3
DOI
出版物ステータスPublished - 2013 3 1

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ASJC Scopus subject areas

  • General

これを引用

Sakaguchi, K., Shimizu, T., Horaguchi, S., Sekine, H., Yamato, M., Umezu, M., & Okano, T. (2013). In vitro engineering of vascularized tissue surrogates. Scientific reports, 3, [1316]. https://doi.org/10.1038/srep01316