In vitro reconstitution of the Escherichia coli 70S ribosome with a full set of recombinant ribosomal proteins

Ryo Aoyama, Keiko Masuda, Masaru Shimojo, Takashi Kanamori, Takuya Ueda, Yoshihiro Shimizu*

*この研究の対応する著者

研究成果: Article査読

抄録

Many studies of the reconstitution of the Escherichia coli small ribosomal subunit from its individual molecular parts have been reported, but contrastingly, similar studies of the large ribosomal subunit have not been well performed to date. Here, we describe protocols for preparing the 33 ribosomal proteins of the E. coli 50S subunit and demonstrate successful reconstitution of a functionally active 50S particle that can perform protein synthesis in vitro. We also successfully reconstituted both ribosomal subunits (30S and 50S) and 70S ribosomes using a full set of recombinant ribosomal proteins by integrating our developed method with the previously developed fully recombinant-based integrated synthesis, assembly and translation. The approach described here makes a major contribution to the field of ribosome engineering and could be fundamental to the future studies of ribosome assembly processes.

本文言語English
ページ(範囲)227-237
ページ数11
ジャーナルJournal of biochemistry
171
2
DOI
出版ステータスPublished - 2022 2月 1

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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