In vitro synthesis of the E. coli sec translocon from DNA

Hideaki Matsubayashi, Yutetsu Kuruma, Takuya Ueda

研究成果: Article査読

53 被引用数 (Scopus)

抄録

Difficulties in constructing complex lipid/protein membranes have severely limited the development of functional artificial cells endowed with vital membrane-related functions. The Sec translocon membrane channel, which mediates the insertion of membrane proteins into the plasma membrane, was constructed in the membrane of lipid vesicles through in vitro expression of its component proteins. The components of the Sec translocon were synthesized from their respective genes in the presence of liposomes, thereby bringing about a functional complex. The synthesized E. coli Sec translocon mediated the membrane translocation of single- and multi-span membrane proteins. The successful translocation of a functional peptidase into the liposome lumen further confirmed the proper insertion of the translocon complex. Our results demonstrate the feasible construction of artificial cells, the membranes of which can be functionalized by directly decoding genetic information into membrane functions.

本文言語English
ページ(範囲)7535-7538
ページ数4
ジャーナルAngewandte Chemie - International Edition
53
29
DOI
出版ステータスPublished - 2014 7 14
外部発表はい

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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