Increased resistance to nitric oxide cytotoxicity associated with differentiation of neuroblastoma-glioma hybrid (NG108-15) cells

Koichi Kawahara*, Munetaka Saitoh, Takayuki Nakajima, Hideomi Sato, Motoki Tanaka, Takuro Tojima, Eturo Ito

*この研究の対応する著者

研究成果: Review article査読

9 被引用数 (Scopus)

抄録

Nitric oxide (NO), synthesized by the enzyme nitric oxide synthase (NOS), acts as an intercellular messenger associated with various physiological and pathological events. In this study, we investigated whether there exits a difference in the vulnerability to NO-induced cytotoxicity between undifferentiated and differentiated NG108-15 cells, and if so, the mechanisms responsible for the difference. Following a 7- to 8-day exposure to dibutyryl cAMP (dbcAMP), NG108-15 cells exhibited a neuron-like morphology associated with the expression of the neuronal protein, synaptophysin, and with increased NADPH-d activity. Neuron-like differentiated NG108-15 cells acquired resistance to exogenously applied NO. This increased resistance to NO toxicity in differentiated cells was almost completely cancelled out by inhibiting the activity of superoxide dismutase (SOD), but not by inhibiting the activity of NOS. The present study suggested that the activity of SOD increased in parallel with the activity of NOS associated with differentiation and was crucial for the acquired resistance to NO toxicity in differentiated cells.

本文言語English
ページ(範囲)545-554
ページ数10
ジャーナルFree Radical Research
36
5
DOI
出版ステータスPublished - 2002 5月 1
外部発表はい

ASJC Scopus subject areas

  • 生化学

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