Increased stability of the higher order structure of chicken erythrocyte chromatin: Nanosecond anisotropy studies of intercalated ethidium

Ikuo Ashikawa, Kazuhiko Kinosita, Akira Ikegami, Yoshifumi Nishimura, Masamichi Tsuboi

研究成果: Article

14 被引用数 (Scopus)

抄録

Internal motion of the DNA in chicken erythrocyte chromatin fibers was studied by measurement of the fluorescence anisotropy decay of ethidium intercalated in the linker region. A comparison of the decay curves of the dye in chicken erythrocyte chromatin with those of calf thymus chromatin [Ashikawa, I., Kinosita, K., Jr., Ikegami, A., Nishimura, Y., Tsuboi, M., Watanabe, K., Iso, K., & Nakano, T. (1983) Biochemistry 22, 6018-6026] revealed greater suppression of nucleosome movement in chicken erythrocyte chromatin. Furthermore, the transition of this chromatin to the compact (solenoidal) structure occurred at lower solvent concentrations of Na+ or Mg2+ than those for calf thymus chromatin. These results demonstrated increased stability of the higher order structure (the solenoid) of chicken erythrocyte chromatin, which may be related to the reduction of nuclear activity in the chicken erythrocyte cell. In addition to intact chicken erythrocyte chromatin, we studied the structural transitions of H1-depleted and H1,H5-depleted chromatins. The result indicated that histone H5 of this chromatin stabilizes the higher order structure in the presence of magnesium (or divalent) cation and did not induce the transition in the solution containing only sodium cation.

本文言語English
ページ(範囲)1291-1297
ページ数7
ジャーナルBiochemistry
24
6
出版ステータスPublished - 1985
外部発表はい

ASJC Scopus subject areas

  • Biochemistry

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