Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells

Jong Soo Kim, Tatiana B. Krasieva, Hitoshi Kurumizaka, David J. Chen, A. Malcolm R Taylor, Kyoko Yokomori

    研究成果: Article査読

    201 被引用数 (Scopus)

    抄録

    Damage recognition by repair/checkpoint factors is the critical first step of the DNA damage response. DNA double strand breaks (DSBs) activate checkpoint signaling and are repaired by nonhomologous end-joining (NHEJ) and homologous recombination (HR) pathways. However, in vivo kinetics of the individual factor responses and the mechanism of pathway choice are not well understood. We report cell cycle and time course analyses of checkpoint activation by ataxia-telangiectasia mutated and damage site recruitment of the repair factors in response to laserinduced DSBs. We found that MRN acts as a DNA damage marker, continuously localizing at unrepaired damage sites. Damage recognition by NHEJ factors precedes that of HR factors. HR factor recruitment is not influenced by NHEJ factor assembly and occurs throughout interphase. Damage site retention of NHEJ factors is transient, whereas HR factors persist at unrepaired lesions, revealing unique roles of the two pathways in mammalian cells.

    本文言語English
    ページ(範囲)341-347
    ページ数7
    ジャーナルJournal of Cell Biology
    170
    3
    DOI
    出版ステータスPublished - 2005 9 1

    ASJC Scopus subject areas

    • 細胞生物学

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