Independent flexible motion of submolecular domains of the Ca2+,Mg2+-ATPase of sarcoplasmic reticulum measured by time-resolved fluorescence depolarization of site-specifically attached probes

Sei Ichi Suzuki, Suguru Kawato, Tsutomu Kouyama, Kazuhiko Kinosita, Akira Ikegami, Masao Kawakita

研究成果: Article

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The Ca2+-transporting ATPase of rabbit skeletal muscle sarcoplasmic reticulum was sitespecifically labeled with either N-(1-anilinonaphth-4-yl)maleimide (ANM) or 5-[[(iodoacetamido)-ethyl]amino]naphthalene-1-sulfonate (IAEDANS), and the segmental motion of submolecular domains of the ATPase molecule was examined by means of time-resolved and steady-state fluorescence anisotropy measurements. The ANM-binding domain showed wobbling with a rotational relaxation time φ = 69 ns in the absence of free Ca2+ without any independent wobbling of the ANM moiety. The IAEDANS-binding domain showed a significantly slower wobbling with φ = 190 ns in the absence of Ca2+. The present results demonstrated for the first time that the ATPase molecule is composed of distinct domains whose mobilities are considerably different from each other. The binding of Ca2+ to the transport site increased the segmental motion of ANM-labeled domain, leading to a phi; value of 65 ns. Solubilization of the ANM-labeled SR membranes by deoxycholate led to a further increase in the segmental flexibility (φ = 48 ns in the absence of free Ca2+), indicating that the mobility of the ANM-binding domain was considerably restricted through interaction with the membrane. The mobility of the ANM-binding domain of solubilized ATPase was also increased to some extent upon binding of Ca2+.

元の言語English
ページ(範囲)7734-7740
ページ数7
ジャーナルBiochemistry
28
発行部数19
出版物ステータスPublished - 1989
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry

フィンガープリント Independent flexible motion of submolecular domains of the Ca<sup>2+</sup>,Mg<sup>2+</sup>-ATPase of sarcoplasmic reticulum measured by time-resolved fluorescence depolarization of site-specifically attached probes' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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