Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA

Akihisa Osakabe, Fumiya Adachi, Yasuhiro Arimura, Kazumitsu Maehara, Yasuyuki Ohkawa, Hitoshi Kurumizaka*

*この研究の対応する著者

研究成果: Article査読

13 被引用数 (Scopus)

抄録

DNA methylation occurs on CpG sites and is important to form pericentric heterochromatin domains. The satellite 2 sequence, containing seven CpG sites, is located in the pericentric region of human chromosome 1 and is highly methylated in normal cells. In contrast, the satellite 2 region is reportedly hypomethylated in cancer cells, suggesting that the methylation status may affect the chromatin structure around the pericentric regions in tumours. In this study, we mapped the nucleosome positioning on the satellite 2 sequence in vitro and found that DNA methylation modestly affects the distribution of the nucleosome positioning. The micrococcal nuclease assay revealed that the DNA end flexibility of the nucleosomes changes, depending on the DNA methylation status. However, the structures and thermal stabilities of the nucleosomes are unaffected by DNA methyl-ation. These findings provide new information to understand how DNA methylation functions in regulating pericentric heterochromatin formation and maintenance in normal and malignant cells.

本文言語English
論文番号150128
ジャーナルOpen Biology
5
10
DOI
出版ステータスPublished - 2015 10 7

ASJC Scopus subject areas

  • 神経科学(全般)
  • 免疫学
  • 生化学、遺伝学、分子生物学(全般)

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