Influence of growth phase on bacterial cell electrokinetic characteristics examined by soft particle electrophoresis theory

Hiroshi Hayashi, Hiroshi Seiki, Satoshi Tsuneda*, Akira Hirata, Hiroshi Sasaki

*この研究の対応する著者

研究成果査読

36 被引用数 (Scopus)

抄録

The influence of incubation time on the electrokinetic properties of Escherichia coli, Pseudomonas putida, Alcaligenes faecalis, and Alcaligenes sp., was examined by electrophoretic mobility measurements and the results were discussed based on Ohshima's soft particle theory. The electrophoretic mobility of E. coli plotted against incubation time revealed that the mobility gradually increased from the outset of incubation to 7 h, which corresponded to the exponential growth and early stationary phase, and then decreased. For all strains, electrophoretic mobility leveled off to nonzero values in accordance with the increase in ionic concentration, which was a characteristic feature of soft particles. Soft particle analysis was carried out at the outset of incubation and the time when mobility reached a maximum in order to obtain the spatial charge density (ZN) and cell surface softness (1/λ). ZN at the maximum electrophoretic mobility was more negative than that at the outset of incubation for E. coli, P. putida, and A. faecalis, while the ZN of Alcaligenes sp. almost remained unchanged. 1/λ decreased for E. coli, but increased for Alcaligenes sp. and A. faecalis. These findings indicated that the cell growth phase affects both ZN and 1/λ, although the dependencies of these parameters are unique to each bacterial strain.

本文言語English
ページ(範囲)565-568
ページ数4
ジャーナルJournal of Colloid And Interface Science
264
2
DOI
出版ステータスPublished - 2003 8 15

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 表面、皮膜および薄膜
  • コロイド化学および表面化学

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