Influence of moonlight on mRNA expression patterns of melatonin receptor subtypes in the pineal organ of a tropical fish

Yong Ju Park, Ji Gweon Park, Yuki Takeuchi, Sung Pyo Hur, Young Don Lee, Se Jae Kim, Akihiro Takemura

研究成果: Article

10 引用 (Scopus)

抄録

The goldlined spinefoot, Siganus guttatus, is a lunar-synchronized spawner, which repeatedly releases gametes around the first quarter moon during the reproductive season. A previous study reported that manipulating moonlight brightness at night disrupted synchronized spawning, suggesting involvement of this natural light source in lunar synchronization. The present study examined whether the mRNA expression pattern of melatonin receptor subtypes MT1 and Mel1c in the pineal organ of the goldlined spinefoot is related to moonlight. Real-time quantitative polymerase chain reaction analysis revealed that the abundance of MT1 and Mel1c mRNA at midnight increased during the new moon phase and decreased during the full moon phase. Exposing fish to moonlight intensity during the full moon period resulted in a decrease in Mel1c mRNA abundance within 1h. Fluctuations in the melatonin receptor genes according to changes in the moon phase agreed with those of melatonin levels in the blood. These results indicate that periodic changes in cues from the moon influence melatonin receptor mRNA expression levels. The melatonin-melatonin receptor system may play a role in predicting the moon phase through changes in night brightness.

元の言語English
ページ(範囲)67-70
ページ数4
ジャーナルMarine Genomics
14
DOI
出版物ステータスPublished - 2014
外部発表Yes

Fingerprint

Melatonin Receptors
lunar phase
tropical fish
melatonin
Fishes
Moon
Messenger RNA
receptors
fish
Melatonin
gamete
polymerase chain reaction
Siganus
Melatonin MT1 Receptor
spawning
blood
phase transition
organ
gene
germ cells

ASJC Scopus subject areas

  • Aquatic Science
  • Genetics
  • Medicine(all)

これを引用

Influence of moonlight on mRNA expression patterns of melatonin receptor subtypes in the pineal organ of a tropical fish. / Park, Yong Ju; Park, Ji Gweon; Takeuchi, Yuki; Hur, Sung Pyo; Lee, Young Don; Kim, Se Jae; Takemura, Akihiro.

:: Marine Genomics, 巻 14, 2014, p. 67-70.

研究成果: Article

Park, Yong Ju ; Park, Ji Gweon ; Takeuchi, Yuki ; Hur, Sung Pyo ; Lee, Young Don ; Kim, Se Jae ; Takemura, Akihiro. / Influence of moonlight on mRNA expression patterns of melatonin receptor subtypes in the pineal organ of a tropical fish. :: Marine Genomics. 2014 ; 巻 14. pp. 67-70.
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abstract = "The goldlined spinefoot, Siganus guttatus, is a lunar-synchronized spawner, which repeatedly releases gametes around the first quarter moon during the reproductive season. A previous study reported that manipulating moonlight brightness at night disrupted synchronized spawning, suggesting involvement of this natural light source in lunar synchronization. The present study examined whether the mRNA expression pattern of melatonin receptor subtypes MT1 and Mel1c in the pineal organ of the goldlined spinefoot is related to moonlight. Real-time quantitative polymerase chain reaction analysis revealed that the abundance of MT1 and Mel1c mRNA at midnight increased during the new moon phase and decreased during the full moon phase. Exposing fish to moonlight intensity during the full moon period resulted in a decrease in Mel1c mRNA abundance within 1h. Fluctuations in the melatonin receptor genes according to changes in the moon phase agreed with those of melatonin levels in the blood. These results indicate that periodic changes in cues from the moon influence melatonin receptor mRNA expression levels. The melatonin-melatonin receptor system may play a role in predicting the moon phase through changes in night brightness.",
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T1 - Influence of moonlight on mRNA expression patterns of melatonin receptor subtypes in the pineal organ of a tropical fish

AU - Park, Yong Ju

AU - Park, Ji Gweon

AU - Takeuchi, Yuki

AU - Hur, Sung Pyo

AU - Lee, Young Don

AU - Kim, Se Jae

AU - Takemura, Akihiro

PY - 2014

Y1 - 2014

N2 - The goldlined spinefoot, Siganus guttatus, is a lunar-synchronized spawner, which repeatedly releases gametes around the first quarter moon during the reproductive season. A previous study reported that manipulating moonlight brightness at night disrupted synchronized spawning, suggesting involvement of this natural light source in lunar synchronization. The present study examined whether the mRNA expression pattern of melatonin receptor subtypes MT1 and Mel1c in the pineal organ of the goldlined spinefoot is related to moonlight. Real-time quantitative polymerase chain reaction analysis revealed that the abundance of MT1 and Mel1c mRNA at midnight increased during the new moon phase and decreased during the full moon phase. Exposing fish to moonlight intensity during the full moon period resulted in a decrease in Mel1c mRNA abundance within 1h. Fluctuations in the melatonin receptor genes according to changes in the moon phase agreed with those of melatonin levels in the blood. These results indicate that periodic changes in cues from the moon influence melatonin receptor mRNA expression levels. The melatonin-melatonin receptor system may play a role in predicting the moon phase through changes in night brightness.

AB - The goldlined spinefoot, Siganus guttatus, is a lunar-synchronized spawner, which repeatedly releases gametes around the first quarter moon during the reproductive season. A previous study reported that manipulating moonlight brightness at night disrupted synchronized spawning, suggesting involvement of this natural light source in lunar synchronization. The present study examined whether the mRNA expression pattern of melatonin receptor subtypes MT1 and Mel1c in the pineal organ of the goldlined spinefoot is related to moonlight. Real-time quantitative polymerase chain reaction analysis revealed that the abundance of MT1 and Mel1c mRNA at midnight increased during the new moon phase and decreased during the full moon phase. Exposing fish to moonlight intensity during the full moon period resulted in a decrease in Mel1c mRNA abundance within 1h. Fluctuations in the melatonin receptor genes according to changes in the moon phase agreed with those of melatonin levels in the blood. These results indicate that periodic changes in cues from the moon influence melatonin receptor mRNA expression levels. The melatonin-melatonin receptor system may play a role in predicting the moon phase through changes in night brightness.

KW - Goldlined spinefoot

KW - Lunar cycle

KW - Melatonin

KW - Moonlight

KW - Rabbitfish

KW - Real-time quantitative polymerase chain reaction

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