Influence of Temperature on Glucose Metabolism of Round Spermatids of Rats: temperature/glucose metabolism/spermatids/rat testis

MASAHISA NAKAMURA*, AKIKO FUJIWARA, AKIRA SUZUKI, SHOICHI OKINAGA, KIYOSHI ARAI

*この研究の対応する著者

研究成果: Article査読

抄録

Glucose utilization by spermatids was found to be 17.37±0.37 nmoles/hr/106 cells at 34°C and 28.94±1.12nmoles/hr/106 cells at 40°C. A good parallelism was observed between the increased rate of glucose utilization and lactate production at 40°C. There was no significant change in the levels of glycolytic intermediates in the cells, except for marked accumulations of fructose‐1, 6‐diphosphate, dihydroxyacetone phosphate and glyceraldehyde‐3‐phosphate in the presence of glucose (1 mM). Glucose oxidation in the citrate cycle by spermatids was higher at 40°C than at 34°C, but was never greater than 2% of the overall rate of glucose utilization. In addition, glucose did not prevent decrease of ATP at either 34 or 40°C. The effects of temperature on the activities of 11 glycolytic enzymes were examined. The activities of aldolase and phosphoglyceromutase were similar between 30 and 34°C, but increased markedly at 40°C. The higher temperature increased the Vmax values, without affecting the Kms. The activities of other glycolytic enzymes were similar at the different temperatures. These findings indicate that the increased overall rate of glucose utilization in glycolysis at higher temperature is due to increased Vmax values of aldolase and phosphoglyceromutase.

本文言語English
ページ(範囲)49-56
ページ数8
ジャーナルDevelopment Growth and Differentiation
25
1
DOI
出版ステータスPublished - 1983
外部発表はい

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学

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