Influence of temperature on lactate utilization by round spermatids from rat testes

M. Nakamura, I. Yasumasu, S. Okinaga, K. Arai

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Rotenone-sensitive 14CO2 formation from [14C]lactate and oxygen consumption by round spermatids were found to be greater at elevated temperatures than at 34°C. More than 96% of the total radioactivity of the metabolized [14C]lactate was recovered in the released CO2 and the acid soluble fraction of the cells. There was practically no incorporation of [14C]lactate into the lipid, nucleic acid, and protein fractions. Intracellular level of ATP in spermatids was enhanced in the presence of lactate (20 mM) at 34°C (scrotal temperature), whereas it was decreased at 37°C (body temperature). However, this was reversible when the cells were transferred from the elevated temperature to 34°C. It was also found that oxygen consumption and CO2 production were increased at 34° C by 2,4-dinitrophenol (DNP), but decreased by oligomycin. On the contrary, oligomycin and DNP had no effect on oxygen consumption and 14CO2 formation at the elevated temperature. These findings provide evidence that lactate utilization by spermatids is coupled with oxidative phosphorylation at scrotal temperature, but becomes uncoupled at elevated temperature, although more lactate is consumed.

本文言語English
ページ(範囲)265-272
ページ数8
ジャーナルDevelopment Growth and Differentiation
24
3
出版ステータスPublished - 1982
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学
  • 発生生物学
  • 解剖学

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