Inhibition of filament formation of human Rad51 protein by a small peptide derived from the BRC-motif of the BRCA2 protein

Julian Nomme, Yoshimasa Takizawa, Susan F. Martinez, Axelle Renodon-Cornière, Fabrice Fleury, Pierre Weigel, Ken Ichi Yamamoto, Hitoshi Kurumizaka, Masayuki Takahashi*

*この研究の対応する著者

    研究成果: Article査読

    32 被引用数 (Scopus)

    抄録

    Human Rad51 is a key element of recombinational DNA repair and is related to the resistance of cancer cells to chemo- and radiotherapies. The protein is thus a potential target of anti-cancer treatment. The crystallographic analysis shows that the BRC-motif of the BRCA2 tumor suppressor is in contact with the subunit-subunit interface of Rad51 and could thus prevent filament formation of Rad51. However, biochemical analysis indicates that a BRC-motif peptide of 69 amino acids preferentially binds to the N-terminal part of Rad51. We show experimentally that a short peptide of 28 amino acids derived from the BRC4 motif binds to the subunit-subunit interface and dissociates its filament, both in the presence and absence of DNA, certainly by binding to dissociated monomers. The inhibition is efficient and specific for Rad51: the peptide does not even interact with Rad51 homologs or prevent their interaction with DNA. Neither the N-terminal nor the C-terminal half of the peptide interacts with human Rad51, indicating that both parts are involved in the interaction, as expected from the crystal structure. These results suggest the possibility of developing inhibitors of human Rad51 based on this peptide.

    本文言語English
    ページ(範囲)471-481
    ページ数11
    ジャーナルGenes to Cells
    13
    5
    DOI
    出版ステータスPublished - 2008 5月

    ASJC Scopus subject areas

    • 遺伝学
    • 細胞生物学

    フィンガープリント

    「Inhibition of filament formation of human Rad51 protein by a small peptide derived from the BRC-motif of the BRCA2 protein」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル