Inhibition of hirame rhabdovirus growth by RNA aptamers

S. D. Hwang, N. Midorikawa, P. Punnarak, Yo Kikuchi, H. Kondo, I. Hirono, T. Aoki

研究成果: Article査読

15 被引用数 (Scopus)

抄録

RNA aptamers are artificial nucleic acids that specifically bind to a wide variety of targets. They are an effective tool for pharmaceutical research and development of antiviral agents. Here, we describe four Hirame rhabdovirus (HIRRV)-RNA aptamers (H1, H2, H3 and H4) that we obtained from an in vitro process called the systematic evolution of ligands by exponential enrichment (SELEX). The HIRRV-RNA aptamers specifically bind to HIRRV. Hirame natural embryo (HINAE) cells treated with virus and the RNA aptamer showed a decrease in appearance of cytopathic effect when compared with control (treated only with virus). Rhodovulum sulfidophilum was transformed with genes for the RNA aptamers, and the aptamers were detected in the culture medium, indicating that they were secreted from the cells. Thus, the recombinant R. sulfidophilum might be a powerful tool for the prevention of HIRRV in aquaculture.

本文言語English
ページ(範囲)927-934
ページ数8
ジャーナルJournal of Fish Diseases
35
12
DOI
出版ステータスPublished - 2012 12
外部発表はい

ASJC Scopus subject areas

  • 水圏科学
  • 獣医学(その他)

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