Small segments of tail of Bufo bufo japonicus tadpoles were cultured in medium containing thyroxine (T4) and dibutyryl cyclic AMP (dbcAMP). Like prolactin, the cyclic nycleotide blocked T4-induced shrinkage of tail pieces. Histological study of the segments after 4 days of culture revealed that dbcAMP suppressed degenerative changes induced by T4. The inhibitory effect of prolactin on T4-induced tail regression was promoted by caffeine an inhibitor of adenosine 3',5'-cyclic monophosphate (cyclic AMP)-phosphodiesterase. The effect of prolactin on the level of cyclic AMP in the tail was also studied in vivo. Sixty min after prolactin injection, the cyclic AMP level was 2-3 times the control value. Possible involvement of cyclic AMP in the action of prolactin, which blocks tail resorption induced by T4, was discussed.
|ジャーナル||Development Growth and Differentiation|
|出版物ステータス||Published - 1979|
ASJC Scopus subject areas
- Cell Biology
- Developmental Biology