抄録
The authors succeeded in raising a single mature haploid Rana rugosa female to the age of 2 years from an egg artificially fertilized with ultraviolet irradiated sperm. In order to discover why this particular haploid individual should survive so long, hydrogen peroxide detoxifying catalase in the liver of this individual and age matched diploids was examined and compared for total activity, temperature stability, and chemical inhibition. Total activity was found to be significantly higher in the haploid frog than in the diploids, suggesting that this particular haploid had a unique system for hydrogen peroxide detoxification which protected the liver against cell death, preventing hepatic failure, and leading to a prolonged survival. Liver catalase from the haploid proved to be more labile to aminotriazole and urea, losing 60-70% of its original activity after 30 min treatment, whereas diploid catalase lost only 40% under the same conditions. Haploid and diploid catalase responded similarly to heat, however. It seems likely that inhibitor-binding sites differ considerably between the catalase of normal diploids and the catalase of this particular haploid, while overall structure is generally similar.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 235-239 |
| ページ数 | 5 |
| ジャーナル | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| 巻 | 119 |
| 発行部数 | 1 |
| DOI | |
| 出版物ステータス | Published - 1998 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Physiology
これを引用
Inhibitor and temperature effect on catalase in the liver of adult diploid and haploid Rana rugosa. / Kashiwagi, Akihiko; Kashiwagi, Keiko; Takase, Minoru; Hanada, Hideki; Yamashita, Masamichi; Naitoh, Tomio; Nakamura, Masahisa.
：: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 巻 119, 番号 1, 1998, p. 235-239.研究成果: Article
}
TY - JOUR
T1 - Inhibitor and temperature effect on catalase in the liver of adult diploid and haploid Rana rugosa
AU - Kashiwagi, Akihiko
AU - Kashiwagi, Keiko
AU - Takase, Minoru
AU - Hanada, Hideki
AU - Yamashita, Masamichi
AU - Naitoh, Tomio
AU - Nakamura, Masahisa
PY - 1998
Y1 - 1998
N2 - The authors succeeded in raising a single mature haploid Rana rugosa female to the age of 2 years from an egg artificially fertilized with ultraviolet irradiated sperm. In order to discover why this particular haploid individual should survive so long, hydrogen peroxide detoxifying catalase in the liver of this individual and age matched diploids was examined and compared for total activity, temperature stability, and chemical inhibition. Total activity was found to be significantly higher in the haploid frog than in the diploids, suggesting that this particular haploid had a unique system for hydrogen peroxide detoxification which protected the liver against cell death, preventing hepatic failure, and leading to a prolonged survival. Liver catalase from the haploid proved to be more labile to aminotriazole and urea, losing 60-70% of its original activity after 30 min treatment, whereas diploid catalase lost only 40% under the same conditions. Haploid and diploid catalase responded similarly to heat, however. It seems likely that inhibitor-binding sites differ considerably between the catalase of normal diploids and the catalase of this particular haploid, while overall structure is generally similar.
AB - The authors succeeded in raising a single mature haploid Rana rugosa female to the age of 2 years from an egg artificially fertilized with ultraviolet irradiated sperm. In order to discover why this particular haploid individual should survive so long, hydrogen peroxide detoxifying catalase in the liver of this individual and age matched diploids was examined and compared for total activity, temperature stability, and chemical inhibition. Total activity was found to be significantly higher in the haploid frog than in the diploids, suggesting that this particular haploid had a unique system for hydrogen peroxide detoxification which protected the liver against cell death, preventing hepatic failure, and leading to a prolonged survival. Liver catalase from the haploid proved to be more labile to aminotriazole and urea, losing 60-70% of its original activity after 30 min treatment, whereas diploid catalase lost only 40% under the same conditions. Haploid and diploid catalase responded similarly to heat, however. It seems likely that inhibitor-binding sites differ considerably between the catalase of normal diploids and the catalase of this particular haploid, while overall structure is generally similar.
KW - Catalase activity
KW - Catalase chemical inhibition
KW - Catalase temperature stability
KW - HO removal mechanisms
KW - Haploid survival
KW - Haploid syndrome
KW - Peroxisomal enzymes
KW - Programmed cell death
KW - Rana rugosa
UR - http://www.scopus.com/inward/record.url?scp=0031911916&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031911916&partnerID=8YFLogxK
U2 - 10.1016/S0305-0491(97)00321-0
DO - 10.1016/S0305-0491(97)00321-0
M3 - Article
C2 - 9530823
AN - SCOPUS:0031911916
VL - 119
SP - 235
EP - 239
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 1
ER -