Inhibitory effect of Lysichiton camtschatcense extracts on Fe 2+/ascorbate-induced lipid peroxidation in rat kidney and brain homogenates

Hirokatsu Takatsu, Masahiro Ogata, Koji Fukui, Tadahiro Takeda, Toyoshige Endo, Shiro Urano

研究成果: Article

1 引用 (Scopus)

抄録

Lysichiton camtschatcense is a well-known plant in Japan where it has been used as a traditional medicine by the "Ainu" people for the treatment of acute nephritis. It is presumed that L. camtschatcense has an inhibitory effect against nephritis caused by reactive oxygen species (ROS) owing to its antioxidant activities. Consequently, the antioxidant effect of L. camtschatcense extracts was assessed against Fe2+/ascorbic acid (AsA)-induced lipid peroxidation in rat kidney and brain homogenates. The antioxidant effect of the chloroform extract (CE) was more potent than that of the methanol extract (ME) for both homogenates. The antioxidant effect of both extracts was similar to those of α-tocopherol, a lipid-soluble antioxidant, and glutathione (GSH), a water-soluble antioxidant, which were used as reference compounds. Although CE showed a low radical-scavenging effect for superoxide anion radicals (O 2 •- ) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, assessed by using an electron spin resonance (ESR) method, hydroxyl radicals (•OH) were markedly scavenged by more than 80%. On the other hand, ME showed more significant scavenging effect for DPPH radicals and O 2 •- than CE. These results suggest that the inhibitory effects of the L. camtschatcense extract on lipid peroxidation in rat kidney and brain are based on its high radical-scavenging effect against •OH, O 2 •- , and lipid-derived radicals generated from the cell membrane.

元の言語English
ページ(範囲)364-367
ページ数4
ジャーナルJournal of Natural Medicines
63
発行部数3
DOI
出版物ステータスPublished - 2009 7
外部発表Yes

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Araceae
Lipid Peroxidation
Rats
Brain
Antioxidants
Kidney
Lipids
Scavenging
Chloroform
Nephritis
Superoxides
Methanol
Tocopherols
Electron Spin Resonance Spectroscopy
Traditional Medicine
Cell membranes
Hydroxyl Radical
Ascorbic Acid
Medicine
Glutathione

ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Complementary and alternative medicine

これを引用

Inhibitory effect of Lysichiton camtschatcense extracts on Fe 2+/ascorbate-induced lipid peroxidation in rat kidney and brain homogenates. / Takatsu, Hirokatsu; Ogata, Masahiro; Fukui, Koji; Takeda, Tadahiro; Endo, Toyoshige; Urano, Shiro.

:: Journal of Natural Medicines, 巻 63, 番号 3, 07.2009, p. 364-367.

研究成果: Article

Takatsu, Hirokatsu ; Ogata, Masahiro ; Fukui, Koji ; Takeda, Tadahiro ; Endo, Toyoshige ; Urano, Shiro. / Inhibitory effect of Lysichiton camtschatcense extracts on Fe 2+/ascorbate-induced lipid peroxidation in rat kidney and brain homogenates. :: Journal of Natural Medicines. 2009 ; 巻 63, 番号 3. pp. 364-367.
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AB - Lysichiton camtschatcense is a well-known plant in Japan where it has been used as a traditional medicine by the "Ainu" people for the treatment of acute nephritis. It is presumed that L. camtschatcense has an inhibitory effect against nephritis caused by reactive oxygen species (ROS) owing to its antioxidant activities. Consequently, the antioxidant effect of L. camtschatcense extracts was assessed against Fe2+/ascorbic acid (AsA)-induced lipid peroxidation in rat kidney and brain homogenates. The antioxidant effect of the chloroform extract (CE) was more potent than that of the methanol extract (ME) for both homogenates. The antioxidant effect of both extracts was similar to those of α-tocopherol, a lipid-soluble antioxidant, and glutathione (GSH), a water-soluble antioxidant, which were used as reference compounds. Although CE showed a low radical-scavenging effect for superoxide anion radicals (O 2 •- ) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, assessed by using an electron spin resonance (ESR) method, hydroxyl radicals (•OH) were markedly scavenged by more than 80%. On the other hand, ME showed more significant scavenging effect for DPPH radicals and O 2 •- than CE. These results suggest that the inhibitory effects of the L. camtschatcense extract on lipid peroxidation in rat kidney and brain are based on its high radical-scavenging effect against •OH, O 2 •- , and lipid-derived radicals generated from the cell membrane.

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