Insulin-like growth factor-I stimulates endothelin-3 secretion from rat anterior pituitary cells in primary culture

Hirokazu Matsumoto*, Nobuhiro Suzuki, Kunio Shiota, Kinji Inoue, Masao Tsuda, Masahiko Fujino

*この研究の対応する著者

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Since we found relatively high concentrations of immunoreactive (ir-) ET-3 in the rat pituitary gland (190 pg/g tissue), we have investigated the possible ET-3 secretion from the primary culture of anterior pituitary cells and the effects of various growth factors on the ET-3 secretion. The ir-ET-3 was detected in the incubation medium within 2 h, and 24 h of culture attained the concentrations of 1.15 ± 0.26 pg/well/6 × 105 cells. The ir-ET-3 secretion was stimulated by insulin, insulin like growth factor-II (IGF-II), and most effectively by insulin like growth factor-I (IGF-I) in a dose- and time-dependent manner, whereas the production of ir-ET-1 and ir-big ET-1 was slightly inhibited by IGF-I and IGF-II. In reverse-phase HPLC, the ir-ET-3 released into the culture media showed identical retention time with authentic ET-3. Although ir-ET-1 and ir-big ET-1 secretion was stimulated by transforming growth factor-β (TGF-β), ir-ET-3 secretion was inhibited. These results indicate that the anterior pituitary cells secrete ET-3 and the secretion is stimulated by IGF-I.

本文言語English
ページ(範囲)661-668
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
172
2
DOI
出版ステータスPublished - 1990 10月 30
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 分子生物学

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