Integration in a multilayer microfluidic chip of 8 parallel cell sorters with flow control by sol-gel transition of thermoreversible gelation polymer

Hirokazu Sugino, Takahiro Arakawa, Yuki Nara, Yoshitaka Shirasaki, Kazuto Ozaki, Shuichi Shoji, Takashi Funatsu*

*この研究の対応する著者

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Microfluidic systems have significant implications in the field of cell separation since they could provide platforms with inexpensive, disposable and sterile structures. Here, we present a novel strategy to integrate microfluidic sorters into a single chip for high throughput sorting. Our parallel sorter consists of a microfluidic chip with a three-dimensional channel network that utilizes flow switching by a heat-induced sol-gel transition of thermoreversible gelation polymer. The 8 parallel sheathed sample flows were realized by injecting sample and buffer solutions into only 2 inlets. The sheathed flows enabled disposal of unwanted sample waste without laser irradiation, and collection of wanted sample upon irradiation. As an application of the sorter, two kinds of fluorescent microspheres were separated with recovery ratio and purity of 70% or 90% at throughputs of about 100 or 20 particles per second, respectively. Next, Escherichia coli cells expressing green fluorescent protein were separated from those expressing DsRed with recovery ratio and purity of 90% at a throughput of about 20 cells per second.

本文言語English
ページ(範囲)2559-2565
ページ数7
ジャーナルLab on a Chip
10
19
DOI
出版ステータスPublished - 2010 10月 7

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 生化学
  • 化学 (全般)
  • 生体医工学

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