Integration of pillar array columns into a gradient elution system for pressure-driven liquid chromatography

Yanting Song, Masao Noguchi, Katsuya Takatsuki, Tetsushi Sekiguchi, Jun Mizuno, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda*

*この研究の対応する著者

研究成果: Article査読

36 被引用数 (Scopus)

抄録

A gradient elution system for pressure-driven liquid chromatography (LC) on a chip was developed for carrying out faster and more efficient chemical analyses. Through computational fluid dynamics simulations and an experimental study, we found that the use of a cross-Tesla structure with a 3 mm mixing length was effective for mixing two liquids. A gradient elution system using a cross-Tesla mixer was fabricated on a 20 mm × 20 mm silicon chip with a separation channel of pillar array columns and a sample injection channel. A mixed solution of water and fluorescein in methanol was delivered to the separation channel 7 s after the gradient program had been started. Then, the fluorescence intensity increased gradually with the increasing ratio of fluorescein, which showed that the gradient elution worked well. Under the gradient elution condition, the retention times of two coumarin dyes decreased with the gradient time. When the gradient time was 30 s, the analysis could be completed in 30 s, which was only half the time required compared to that required for an isocratic elution. Fluorescent derivatives of aliphatic amines were successfully separated within 110 s. The results show that the proposed system is promising for the analyses of complex biological samples.

本文言語English
ページ(範囲)4739-4745
ページ数7
ジャーナルAnalytical Chemistry
84
11
DOI
出版ステータスPublished - 2012 6月 5

ASJC Scopus subject areas

  • 分析化学

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