抄録
Adrenaline reacts with three types of adrenergic receptors, α1, α2 and β-adrenergic receptors (ARs), inducing many physiological events including exocytosis. Although adrenaline has been shown to induce glucagon-like peptide-1 (GLP-1) secretion from intestinal L cells, the precise molecular mechanism by which adrenaline regulates GLP-1 secretion remains unknown. Here we show by live cell imaging that all types of adrenergic receptors are stimulated by adrenaline in enteroendocrine L cell line GLUTag cells and are involved in GLP-1 exocytosis. We performed RT-PCR analysis and found that α1B-, α2A-, α2B-, and β1-ARs were expressed in GLUTag cells. Application of adrenaline induced a significant increase of intracellular Ca<sup>2+</sup> and cAMP concentration ([Ca<sup>2+</sup>]<inf>i</inf> and [cAMP]<inf>i</inf>, respectively), and GLP-1 exocytosis in GLUTag cells. Blockade of α1-AR inhibited adrenaline-induced [Ca<sup>2+</sup>]<inf>i</inf> increase and exocytosis but not [cAMP]<inf>i</inf> increase, while blockade of β1-AR inhibited adrenaline-induced [cAMP]<inf>i</inf> increase and exocytosis but not [Ca<sup>2+</sup>]<inf>i</inf> increase. Furthermore, overexpression of α2A-AR suppressed the adrenaline-induced [cAMP]<inf>i</inf> increase and exocytosis. These results suggest that the fine-turning of GLP-1 secretion from enteroendocrine L cells is established by the balance between α1-, α2-, and β-ARs activation.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 1053-1058 |
| ページ数 | 6 |
| ジャーナル | Biochemical and Biophysical Research Communications |
| 巻 | 460 |
| 発行部数 | 4 |
| DOI | |
| 出版物ステータス | Published - 2015 5 12 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
- Medicine(all)
これを引用
Integrative function of adrenaline receptors for glucagon-like peptide-1 exocytosis in enteroendocrine L cell line GLUTag. / Harada, Kazuki; Kitaguchi, Tetsuya; Tsuboi, Takashi.
：: Biochemical and Biophysical Research Communications, 巻 460, 番号 4, 12.05.2015, p. 1053-1058.研究成果: Article
}
TY - JOUR
T1 - Integrative function of adrenaline receptors for glucagon-like peptide-1 exocytosis in enteroendocrine L cell line GLUTag
AU - Harada, Kazuki
AU - Kitaguchi, Tetsuya
AU - Tsuboi, Takashi
PY - 2015/5/12
Y1 - 2015/5/12
N2 - Adrenaline reacts with three types of adrenergic receptors, α1, α2 and β-adrenergic receptors (ARs), inducing many physiological events including exocytosis. Although adrenaline has been shown to induce glucagon-like peptide-1 (GLP-1) secretion from intestinal L cells, the precise molecular mechanism by which adrenaline regulates GLP-1 secretion remains unknown. Here we show by live cell imaging that all types of adrenergic receptors are stimulated by adrenaline in enteroendocrine L cell line GLUTag cells and are involved in GLP-1 exocytosis. We performed RT-PCR analysis and found that α1B-, α2A-, α2B-, and β1-ARs were expressed in GLUTag cells. Application of adrenaline induced a significant increase of intracellular Ca2+ and cAMP concentration ([Ca2+]i and [cAMP]i, respectively), and GLP-1 exocytosis in GLUTag cells. Blockade of α1-AR inhibited adrenaline-induced [Ca2+]i increase and exocytosis but not [cAMP]i increase, while blockade of β1-AR inhibited adrenaline-induced [cAMP]i increase and exocytosis but not [Ca2+]i increase. Furthermore, overexpression of α2A-AR suppressed the adrenaline-induced [cAMP]i increase and exocytosis. These results suggest that the fine-turning of GLP-1 secretion from enteroendocrine L cells is established by the balance between α1-, α2-, and β-ARs activation.
AB - Adrenaline reacts with three types of adrenergic receptors, α1, α2 and β-adrenergic receptors (ARs), inducing many physiological events including exocytosis. Although adrenaline has been shown to induce glucagon-like peptide-1 (GLP-1) secretion from intestinal L cells, the precise molecular mechanism by which adrenaline regulates GLP-1 secretion remains unknown. Here we show by live cell imaging that all types of adrenergic receptors are stimulated by adrenaline in enteroendocrine L cell line GLUTag cells and are involved in GLP-1 exocytosis. We performed RT-PCR analysis and found that α1B-, α2A-, α2B-, and β1-ARs were expressed in GLUTag cells. Application of adrenaline induced a significant increase of intracellular Ca2+ and cAMP concentration ([Ca2+]i and [cAMP]i, respectively), and GLP-1 exocytosis in GLUTag cells. Blockade of α1-AR inhibited adrenaline-induced [Ca2+]i increase and exocytosis but not [cAMP]i increase, while blockade of β1-AR inhibited adrenaline-induced [cAMP]i increase and exocytosis but not [Ca2+]i increase. Furthermore, overexpression of α2A-AR suppressed the adrenaline-induced [cAMP]i increase and exocytosis. These results suggest that the fine-turning of GLP-1 secretion from enteroendocrine L cells is established by the balance between α1-, α2-, and β-ARs activation.
KW - Adrenaline
KW - Adrenergic receptors
KW - Enteroendocrine L cell
KW - Exocytosis
KW - Glucagon-like peptide-1
UR - http://www.scopus.com/inward/record.url?scp=84937761883&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84937761883&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2015.03.151
DO - 10.1016/j.bbrc.2015.03.151
M3 - Article
C2 - 25843795
AN - SCOPUS:84937761883
VL - 460
SP - 1053
EP - 1058
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -