Interaction between Ca2+ and dipalmitoylphosphatidylcholine membranes. II. Fluorescence anisotropy study

Ryoichi Kataoka*, Shuji Aruga, Shigeki Mitaku, Kazuhiko Kinosita, Akira Ikegami

*この研究の対応する著者

研究成果: Article査読

33 被引用数 (Scopus)

抄録

The effect of Ca2+ on the molecular mobility in dipalmitoylphosphatidylcholine membranes was studied by steady-state and time-resolved measurements of fluorescence anisotropy. The fluorescence anisotropy decay of 1,6-diphenyl-1,3,5-hexatriene in the hydrocarbon region indicated that the free volume of molecular rotation became more restricted when the Ca2+ concentration was increased. The decrease of the molecular mobility was observed from 1 mM Ca2+, at which the number of bound Ca2+ is much less than that of the total lipid molecules. A distinct difference between Ca2+ and Mg2+ effects suggested that the change in various membrane properties was induced by the binding of these ions. From these results we propose a long-range attractive interaction between bound Ca2+ and the polar head groups of distant phosphatidylcholine molecules.

本文言語English
ページ(範囲)277-284
ページ数8
ジャーナルBiophysical Chemistry
21
3-4
DOI
出版ステータスPublished - 1985
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 物理化学および理論化学

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