Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA.

L. A. Benkowski, C. Takemoto, G. Ott, M. Beikman, T. Ueda, K. Watanabe, M. Sprinzl, L. L. Spremulli

研究成果: Article査読

6 被引用数 (Scopus)

抄録

The interaction between the bovine mitochondrial translational elongation factor Tu.Ts complex (EF-Tu.Tsmt) and aminoacyl-tRNA has been investigated using a nuclease protection assay and fluorescence enhancement of [AEDANS-s2C]Tyr-tRNA(Tyr). The equilibrium dissociation constant, Kd, for the EF-Tu.Tsmt:GTP:E. coli Phe-tRNA complex is approximately 50 nM. A similar binding constant (30 nM) is obtained using bovine mitochondrial Phe-tRNA. The equilibrium binding constant for the EF-Tu.Tsmt:GTP:yeast [AEDANS-s2C]Tyr-tRNA(Tyr) complex is approximately 4 nM when determined using the fluorescence enhancement assay.

本文言語English
ページ(範囲)163-166
ページ数4
ジャーナルNucleic acids symposium series
33
出版ステータスPublished - 1995
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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