Interaction of starfish gonadotropin with its receptor: Effect of chimeric relaxin-like gonad-stimulating peptides

Masatoshi Mita, Keitaro Nakamura, Kazuyoshi Tsutsui, Hidekazu Katayama

    研究成果: Article

    抄録

    A relaxin-like gonad-stimulating peptide (RGP) of starfish Patiria (Asterina) pectinifera is the first identified invertebrate gonadotropin for final gamete maturation. Recently, we found three orthologs of RGP in the class Asteroida; PpeRGP in P. pectinifera, AamRGP in Asterias amurensis, and AjaRGP in Aphelasterias japonica. In this study, nine kinds of RGP derivatives with exchanged each A- and B-chain were synthesized chemically to analyze the interaction of RGP with its receptor. Among these RGP derivatives, PpeRGP and its chimeric RGPs with B-chains from AamRGP or AjaRGP could induce oocyte maturation and ovulation in P. pectinifera ovaries. In contrast, other RGP derivatives were failed to induce spawning in P. pectinifera ovaries. Circular dichroism spectra of PpeRGP were similar to those of chimeric RGPs with the B-chains from AamRGP or AjaRGP. Furthermore, the predicted three-dimensional structure models of the B-chains from RGP derivatives have almost the same conformation. These findings suggest that the B-chain of PpeRGP is involved in binding to its receptor. Thus, it is likely that the A-chain of AamRGP or AjaRGP disturbs the binding of the PpeRGP B-chain to its receptor.

    元の言語English
    ページ(範囲)30-36
    ページ数7
    ジャーナルGeneral and Comparative Endocrinology
    276
    DOI
    出版物ステータスPublished - 2019 5 15

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    relaxin
    Starfish
    Relaxin
    Asteroidea
    Gonads
    gonadotropins
    Gonadotropins
    gonads
    peptides
    Peptides
    receptors
    Ovary
    Asterina
    Asterina pectinifera
    Asterias
    circular dichroism spectroscopy
    Invertebrates
    Circular Dichroism
    Ovulation
    Germ Cells

    Keywords

      ASJC Scopus subject areas

      • Animal Science and Zoology
      • Endocrinology

      これを引用

      Interaction of starfish gonadotropin with its receptor : Effect of chimeric relaxin-like gonad-stimulating peptides. / Mita, Masatoshi; Nakamura, Keitaro; Tsutsui, Kazuyoshi; Katayama, Hidekazu.

      :: General and Comparative Endocrinology, 巻 276, 15.05.2019, p. 30-36.

      研究成果: Article

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      abstract = "A relaxin-like gonad-stimulating peptide (RGP) of starfish Patiria (Asterina) pectinifera is the first identified invertebrate gonadotropin for final gamete maturation. Recently, we found three orthologs of RGP in the class Asteroida; PpeRGP in P. pectinifera, AamRGP in Asterias amurensis, and AjaRGP in Aphelasterias japonica. In this study, nine kinds of RGP derivatives with exchanged each A- and B-chain were synthesized chemically to analyze the interaction of RGP with its receptor. Among these RGP derivatives, PpeRGP and its chimeric RGPs with B-chains from AamRGP or AjaRGP could induce oocyte maturation and ovulation in P. pectinifera ovaries. In contrast, other RGP derivatives were failed to induce spawning in P. pectinifera ovaries. Circular dichroism spectra of PpeRGP were similar to those of chimeric RGPs with the B-chains from AamRGP or AjaRGP. Furthermore, the predicted three-dimensional structure models of the B-chains from RGP derivatives have almost the same conformation. These findings suggest that the B-chain of PpeRGP is involved in binding to its receptor. Thus, it is likely that the A-chain of AamRGP or AjaRGP disturbs the binding of the PpeRGP B-chain to its receptor.",
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      T2 - Effect of chimeric relaxin-like gonad-stimulating peptides

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      AU - Nakamura, Keitaro

      AU - Tsutsui, Kazuyoshi

      AU - Katayama, Hidekazu

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      AB - A relaxin-like gonad-stimulating peptide (RGP) of starfish Patiria (Asterina) pectinifera is the first identified invertebrate gonadotropin for final gamete maturation. Recently, we found three orthologs of RGP in the class Asteroida; PpeRGP in P. pectinifera, AamRGP in Asterias amurensis, and AjaRGP in Aphelasterias japonica. In this study, nine kinds of RGP derivatives with exchanged each A- and B-chain were synthesized chemically to analyze the interaction of RGP with its receptor. Among these RGP derivatives, PpeRGP and its chimeric RGPs with B-chains from AamRGP or AjaRGP could induce oocyte maturation and ovulation in P. pectinifera ovaries. In contrast, other RGP derivatives were failed to induce spawning in P. pectinifera ovaries. Circular dichroism spectra of PpeRGP were similar to those of chimeric RGPs with the B-chains from AamRGP or AjaRGP. Furthermore, the predicted three-dimensional structure models of the B-chains from RGP derivatives have almost the same conformation. These findings suggest that the B-chain of PpeRGP is involved in binding to its receptor. Thus, it is likely that the A-chain of AamRGP or AjaRGP disturbs the binding of the PpeRGP B-chain to its receptor.

      KW - Chimeric derivatives

      KW - Interaction with receptor

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      KW - Species specificity

      KW - Starfish gonadotropin

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