Interaction of Tomato mosaic virus movement protein with tobacco RIO kinase

Kuniaki Yoshioka, Yasuhiko Matsushita, Masahiro Kasahara, Ken Ichi Konagaya, Hiroshi Nyunoya*

*この研究の対応する著者

研究成果: Article査読

30 被引用数 (Scopus)

抄録

Tomato mosaic virus (ToMV) has a regulatory gene encoding a movement protein (MP) that is involved in the cell-to-cell movement of viral RNA through plasmodesmata. To identify the host cell factors interacting with ToMV MP, we used a recombinant MP probe to isolate cDNA clones from a phage expression library of Nicotiana tabacum by a far-Western screening method. One of the cDNA clones encoded an MP-interacting protein, MIP-T7, homologous to the yeast novel protein kinase, Rio1p. We isolated a full-length cDNA by RT-PCR. The putative gene product was designated NtRIO, and shared 33 and 73% amino acid identity with yeast and Arabidopsis RIO kinases, respectively. In vitro analyses using recombinant proteins showed that NtRIO also interacted with a different MP derived from Cucumber mosaic virus. NtRIO had autophosphorylation activity and phosphorylated ToMV MP. Addition of recombinant tobacco casein kinase 2 resulted in a marked increase in the phosphorylation of NtRIO. The interaction between NtRIO and ToMV MP was inhibited by phosphorylation of NtRIO.

本文言語English
ページ(範囲)223-229
ページ数7
ジャーナルMolecules and Cells
17
2
出版ステータスPublished - 2004 4月 30
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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