Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow

Susumu Kudo, Makoto Hosobuchi, Shogo Kuretoko, Tateki Sumii, Tomoya Shimada, Marie Terada, Kazuo Tanishita

研究成果: Article

抄録

A phenomenon has been observed in which intracellular Ca 2+ concentration in endothelial cells increases upon application of shear stress (Ca 2+ response). It is therefore assumed that Ca 2+ is the second messenger in the transfer of shear stress stimulation into cells. The Ca 2+ response is also known to spread to surrounding cells (Ca 2+ wave). We investigated the effects on Ca 2+ wave among cultured bovine aorta endothelial cells (BAECs) upon inhibiting the main intercellular signaling pathways, such as gap junction and paracrine pathways by inducing Ca 2+ wave using D-myo-inositol 1,4,5-trisphosphate, P4(5)-(l-(2-nitrophenyl)ethyl) ester trisodium salt (Caged IP 3) due to an intracellular IP 3 elevation. In addition, we investigated the Ca 2+ wave among BAECs under shear stress loading. Using Caged IP 3, local release of ATP from BAEC induced Ca 2+ wave. The Ca 2+ wave was inhibited by the inhibitors of paracrine pathways. Furthermore, the Ca 2+ response spread in the direction of the downstream under shear flow. These results suggest that paracrine pathway is dominant in both of flow and no flow conditions.

元の言語English
ページ(範囲)2431-2441
ページ数11
ジャーナルNihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
77
発行部数784
DOI
出版物ステータスPublished - 2011
外部発表Yes

Fingerprint

Endothelial cells
shear stress
Shear stress
aorta
cells
inositols
adenosine triphosphate
Adenosinetriphosphate
Shear flow
stimulation
shear flow
inhibitors
esters
Esters
Salts
salts

ASJC Scopus subject areas

  • Mechanical Engineering
  • Condensed Matter Physics

これを引用

Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow. / Kudo, Susumu; Hosobuchi, Makoto; Kuretoko, Shogo; Sumii, Tateki; Shimada, Tomoya; Terada, Marie; Tanishita, Kazuo.

:: Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B, 巻 77, 番号 784, 2011, p. 2431-2441.

研究成果: Article

Kudo, Susumu ; Hosobuchi, Makoto ; Kuretoko, Shogo ; Sumii, Tateki ; Shimada, Tomoya ; Terada, Marie ; Tanishita, Kazuo. / Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow. :: Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B. 2011 ; 巻 77, 番号 784. pp. 2431-2441.
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AU - Terada, Marie

AU - Tanishita, Kazuo

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