Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow

Susumu Kudo, Makoto Hosobuchi, Shogo Kuretoko, Tateki Sumii, Tomoya Shimada, Marie Terada, Kazuo Tanishita

研究成果: Article査読

抄録

A phenomenon has been observed in which intracellular Ca 2+ concentration in endothelial cells increases upon application of shear stress (Ca 2+ response). It is therefore assumed that Ca 2+ is the second messenger in the transfer of shear stress stimulation into cells. The Ca 2+ response is also known to spread to surrounding cells (Ca 2+ wave). We investigated the effects on Ca 2+ wave among cultured bovine aorta endothelial cells (BAECs) upon inhibiting the main intercellular signaling pathways, such as gap junction and paracrine pathways by inducing Ca 2+ wave using D-myo-inositol 1,4,5-trisphosphate, P4(5)-(l-(2-nitrophenyl)ethyl) ester trisodium salt (Caged IP 3) due to an intracellular IP 3 elevation. In addition, we investigated the Ca 2+ wave among BAECs under shear stress loading. Using Caged IP 3, local release of ATP from BAEC induced Ca 2+ wave. The Ca 2+ wave was inhibited by the inhibitors of paracrine pathways. Furthermore, the Ca 2+ response spread in the direction of the downstream under shear flow. These results suggest that paracrine pathway is dominant in both of flow and no flow conditions.

本文言語English
ページ(範囲)2431-2441
ページ数11
ジャーナルNihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
77
784
DOI
出版ステータスPublished - 2011
外部発表はい

ASJC Scopus subject areas

  • 機械工学
  • 凝縮系物理学

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