A putative transcriptional coactivator KELP of . Arabidopsis thaliana and its homolog of . Brassica campestris (BcKELP) can bind the movement protein (MP) of . Tomato mosaic virus (ToMV) . in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic . Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected . Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.
ASJC Scopus subject areas
- Plant Science