Interlaboratory studies on in vitro test methods for estimating in vivo resorption of calcium phosphate ceramics

Atsuo Ito*, Yu Sogo, Atsushi Yamazaki, Mamoru Aizawa, Akiyoshi Osaka, Satoshi Hayakawa, Masanori Kikuchi, Kimihiro Yamashita, Yumi Tanaka, Mika Tadokoro, Lídia Ágata De Sena, Fraser Buchanan, Hajime Ohgushi, Marc Bohner

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

A potential standard method for measuring the relative dissolution rate to estimate the resorbability of calcium-phosphate-based ceramics is proposed. Tricalcium phosphate (TCP), magnesium-substituted TCP (MgTCP) and zinc-substituted TCP (ZnTCP) were dissolved in a buffer solution free of calcium and phosphate ions at pH 4.0, 5.5 or 7.3 at nine research centers. Relative values of the initial dissolution rate (relative dissolution rates) were in good agreement among the centers. The relative dissolution rate coincided with the relative volume of resorption pits of ZnTCP in vitro. The relative dissolution rate coincided with the relative resorbed volume in vivo in the case of comparison between microporous MgTCPs with different Mg contents and similar porosity. However, the relative dissolution rate was in poor agreement with the relative resorbed volume in vivo in the case of comparison between microporous TCP and MgTCP due to the superimposition of the Mg-mediated decrease in TCP solubility on the Mg-mediated increase in the amount of resorption. An unambiguous conclusion could not be made as to whether the relative dissolution rate is predictive of the relative resorbed volume in vivo in the case of comparison between TCPs with different porosity. The relative dissolution rate may be useful for predicting the relative amount of resorption for calcium-phosphate-based ceramics having different solubility under the condition that the differences in the materials compared have little impact on the resorption process such as the number and activity of resorbing cells. Statement of significance The evaluation and subsequent optimization of the resorbability of calcium phosphate are crucial in the use of resorbable calcium phosphates. Although the resorbability of calcium phosphates has usually been evaluated in vivo, establishment of a standard in vitro method that can predict in vivo resorption is beneficial for accelerating development and commercialization of new resorbable calcium phosphate materials as well as reducing use of animals. However, there are only a few studies to propose such an in vitro method within which direct comparison was carried out between in vitro and in vivo resorption. We propose here an in vitro method based on measuring dissolution rate. The efficacy and limitations of the method were evaluated by international round-robin tests as well as comparison with in vivo resorption studies for future standardization. This study was carried out as one of Versailles Projects on Advanced Materials and Standards (VAMAS).

本文言語English
ページ(範囲)347-355
ページ数9
ジャーナルActa Biomaterialia
25
DOI
出版ステータスPublished - 2015 10 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生体材料
  • 生化学
  • 生体医工学
  • 分子生物学

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