Interleukin‐1–inhibitory IgG in sera from some patients with rheumatoid arthritis

Hiroshi Suzuki, Takao Akama, Masato Okane, Ichiro Kono, Yoshiki Matsui, Kazuhide Yamane, Heihachiro Kashiwagi

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Inhibition of interleukin‐1°aL (IL‐1°aL) activity was detected in 7 of 41 serum samples from patients with rheumatoid arthritis (RA). These 7 sera inhibited not only IL‐1°aL–induced endothelial cell adherence to neutrophils, but also IL‐1°bT–induced endothelial cell adherence, although to a lesser extent. These sera showed no influence on tumor necrosis factor–induced endothelial cell adherence. No inhibitory activity was found in 40 sera from normal control subjects. Studies to further examine these effects included gel filtration analysis of 2 of the RA sera. The inhibitory activity was eluted near Mr 158 kd and above Mr 250 kd. Analysis by protein A affinity chromatography showed that IL‐1–inhibitory activity was present in protein A–binding fractions. Purified IgG (by DE‐52 column chromatography) from RA patients was found to be as potent an inhibitor as the protein A–binding fractions, which suggests that the major inhibitory activity in RA sera is attributable to IgG molecules. These purified IgG molecules also inhibited IL‐1–induced proliferation of mouse thymocytes but did not influence IL‐2–dependent proliferation of the CTLL‐2 murine T cell line. The 7 patients whose sera showed IL‐1–inhibitory activity had mild RA and low titers of rheumatoid factor. The findings, taken together, suggest a possible regulatory role of IL‐1–inhibitory IgG in RA disease activity.

本文言語English
ページ(範囲)1528-1538
ページ数11
ジャーナルArthritis & Rheumatism
32
12
DOI
出版ステータスPublished - 1989 12
外部発表はい

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

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