Investigation of the mechanism of photochemically-induced lysozyme crystallization

Tetsuo Okutsu*, Tohru Taguchi, Jyunya Korenaga, Takashi Kuroiwa, Yu Ishikawa, Shiori Iizuka, Kaori Sugiyama, Hiroaki Horiuchi, Hiroshi Hiratsuka

*この研究の対応する著者

研究成果: Article査読

1 被引用数 (Scopus)

抄録

This work examined the mechanism involved in photoinduced crystallization of a lysozyme, focusing on the structure of the photoproducts that lead to crystal growth. It was determined that dimers formed via linking of tryptophan (Trp) residues are preferentially produced when applying a high-excitation photon fluence. However, these dimers do not lead to crystal growth. A low fluence forms dimers via the combination of tyrosine (Tyr) residues, generating six types of Tyr-Tyr dimers. The dimer that originates from the linking of Tyr53 and Tyr53 has a configuration similar to that of the two adjacent, yet non-linked, lysozyme molecules in the crystal unit cell. The Tyr53-Tyr53 dimer hence acts as a template for lysozyme crystal nucleation.

本文言語English
ページ(範囲)180-186
ページ数7
ジャーナルJournal of Photochemistry and Photobiology A: Chemistry
321
DOI
出版ステータスPublished - 2016 5 1
外部発表はい

ASJC Scopus subject areas

  • 化学 (全般)
  • 化学工学(全般)
  • 物理学および天文学(全般)

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