Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour

Sachiko Taniguchi, Takanobu Nakazawa, Asami Tanimura, Yuji Kiyama, Tohru Tezuka, Ayako M. Watabe, Norikazu Katayama, Kazumasa Yokoyama, Takeshi Inoue, Hiroko Izumi-Nakaseko, Shigeru Kakuta, Katsuko Sudo, Yoichiro Iwakura, Hisashi Umemori, Takafumi Inoue, Niall P. Murphy, Kouichi Hashimoto, Masanobu Kano, Toshiya Manabe, Tadashi Yamamoto

研究成果: Article査読

62 被引用数 (Scopus)

抄録

Major depressive and bipolar disorders are serious illnesses that affect millions of people. Growing evidence implicates glutamate signalling in depression, though the molecular mechanism by which glutamate signalling regulates depression-related behaviour remains unknown. In this study, we provide evidence suggesting that tyrosine phosphorylation of the NMDA receptor, an ionotropic glutamate receptor, contributes to depression-related behaviour. The NR2A subunit of the NMDA receptor is tyrosine-phosphorylated, with Tyr 1325 as its one of the major phosphorylation site. We have generated mice expressing mutant NR2A with a Tyr-1325-Phe mutation to prevent the phosphorylation of this site in vivo. The homozygous knock-in mice show antidepressant-like behaviour in the tail suspension test and in the forced swim test. In the striatum of the knock-in mice, DARPP-32 phosphorylation at Thr 34, which is important for the regulation of depression-related behaviour, is increased. We also show that the Tyr 1325 phosphorylation site is required for Src-induced potentiation of the NMDA receptor channel in the striatum. These data argue that Tyr 1325 phosphorylation regulates NMDA receptor channel properties and the NMDA receptor-mediated downstream signalling to modulate depression-related behaviour.

本文言語English
ページ(範囲)3717-3729
ページ数13
ジャーナルEMBO Journal
28
23
DOI
出版ステータスPublished - 2009 12

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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