Iron feeding optimization and plasmid stability in production of recombinant bacterial magnetic particles by Magnetospirillum magneticum AMB-1 in fed-batch culture

Chendong Yang, Haruko Takeyama, Tadashi Matsunaga

研究成果: Article

16 引用 (Scopus)

抄録

The production of bacterial magnetic particles (BMPs) by recombinant Magnetospirillum magneticum AMB-1 harboring the plasmid pKML was enhanced in pH-regulated fed-batch culture. The addition of fresh nutrients was feedback-controlled as a function of the pH of the culture. The yield of BMPs was optimized by adjusting the rate of ferric iron addition. Feeding ferric quinate at 15.4 μg/min resulted in a BMP yield of 7.5 mg/l, which is the highest yield so far reported. Expression of a plasmid-encoded fusion protein and segregation of the plasmid during bacterial growth were both stable during fed-batch culture. More than 75% of the cells retained the plasmid for 130 h under antibiotic-free conditions. In addition, the fusion protein permitting the display of a specific protein on the BMP surface was also stably expressed.

元の言語English
ページ(範囲)213-216
ページ数4
ジャーナルJournal of Bioscience and Bioengineering
91
発行部数2
DOI
出版物ステータスPublished - 2001
外部発表Yes

Fingerprint

Magnetospirillum
Batch Cell Culture Techniques
Plasmids
Iron
Proteins
Fusion reactions
Quinic Acid
Antibiotics
Nutrients
Bacterial Proteins
Display devices
Feedback
Anti-Bacterial Agents
Food
Growth

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

これを引用

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title = "Iron feeding optimization and plasmid stability in production of recombinant bacterial magnetic particles by Magnetospirillum magneticum AMB-1 in fed-batch culture",
abstract = "The production of bacterial magnetic particles (BMPs) by recombinant Magnetospirillum magneticum AMB-1 harboring the plasmid pKML was enhanced in pH-regulated fed-batch culture. The addition of fresh nutrients was feedback-controlled as a function of the pH of the culture. The yield of BMPs was optimized by adjusting the rate of ferric iron addition. Feeding ferric quinate at 15.4 μg/min resulted in a BMP yield of 7.5 mg/l, which is the highest yield so far reported. Expression of a plasmid-encoded fusion protein and segregation of the plasmid during bacterial growth were both stable during fed-batch culture. More than 75{\%} of the cells retained the plasmid for 130 h under antibiotic-free conditions. In addition, the fusion protein permitting the display of a specific protein on the BMP surface was also stably expressed.",
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AU - Yang, Chendong

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

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KW - Bacterial magnetic particles (BMPs)

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KW - Fed-batch culture

KW - Iron source

KW - Magnetospirillum magneticum AMB-1

KW - Plasmid stability

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